INTRODUCTION 15 



of the egg, while the second factor, the treatment of the egg 

 with the hypertonic solution, was the life-saving factor. Yet 

 this conclusion is unwarranted. It makes no difference whether 

 the treatment of the unfertilized egg with a hypertonic solution 

 follows or precedes the artificial membrane formation (except 

 that the length of time of exposure of the eggs to the hyper- 

 tonic solution differs in both cases). The writer found recently 

 that if once the unfertilized eggs have been treated with a hyper- 

 tonic solution they possess the second factor or substance 

 necessary for development, as long as they are alive. If at 

 any time 24 or 48 hours later they are submitted to the process 

 of artificial membrane formation they will not disintegrate, 

 but develop normally at room temperature. Now if the treat- 

 ment with the hypertonic solution our second factor were 

 really the life-saving agency in fertilization or artificial partheno- 

 genesis, unfertilized eggs which had been treated with a hyper- 

 tonic solution alone (without membrane formation) should live 

 indefinitely. This is, however, not the case. If we treat un- 

 fertilized eggs with a hypertonic solution only, such eggs will 

 die just as fast as unfertilized eggs not treated in this way. 

 If, however, such eggs are subsequently submitted to the 

 process of artificial membrane formation they will develop 

 and live indefinitely. To such eggs the artificial membrane 

 formation becomes a life-saving agency. From these facts 

 we must conclude that both factors of artificial partheno- 

 genesis are required to preserve the life of the egg and prevent 

 its death. 



This conclusion is supported by the fact that for a small 

 percentage of starfish eggs or annelid eggs the mere act of mem- 

 brane formation suffices for development and the prevention of 

 the death of these eggs. We are forced to conclude that such 

 eggs contain or form a substance which prevents the disintegra- 

 tion hastened as a rule by the process of membrane forma- 

 tion; and which in the sea-urchin egg must be produced by the 



