146 ARTIFICIAL PARTHENOGENESIS AND FERTILIZATION 



formation does not proceed so readily. All this harmonizes 

 with the view that it is not the hydrogen ions but only the imdis- 

 sociated acid which diffuses into the egg and that the fatty acids 

 diffuse much more quickly than the mineral acids which may 

 not enter at all. 



We may inquire whether the causation of membrane forma- 

 tion is a chemical or purely a physical effect of the acid. I 

 asked Dr. Hagedoorn to determine the temperature coefficient 

 of membrane formation by means of acids. He found that 

 it is about 2 for a difference of temperature of 10 C., which 

 therefore indicates a chemical reaction. The procedure was 

 to measure the minimum time which eggs must remain in a 

 mixture of 50 c.c. of acid-f-2. 5 c.c. of N/10 butyric acid to cause 

 membrane formation in 95 per cent of the eggs. It turned out 

 that this time is twice as long at 10 C. as at 20 C. This 

 result was confirmed by other determinations at different tem- 

 peratures. 



All the experiments mentioned in this chapter were made on 

 the eggs of the Californian sea-urchin S. purpuratus. 



