APPENDIX. 



General Methods of Preserving and Hardening Tissues and Organs. 1 The 

 fluids which are most commonly used are alcohol, chromic acid solution 

 (1 in 500), picric acid solution (saturated), bichromate of potash solution 

 (2 per cent.), Muller's fluid (bichromate of potash 2 parts ; sulphate of soda 

 1 part; water 100 parts), and bichromate of ammonia (2 per cent.) The 

 following are the methods of hardening the several tissues and organs which 

 are found to give the best general results : 



Tissue or Organ 



Bladder . 



Blood-vessels 



Brain 



Elastic ligament 



Embryos . 



Eye . 



Eyelids 



Ganglia 



Heart 



Injected organs 



Intestine . 



Kidney 



Lachrymal gland 



Larynx 



Liver 



Lung 



Mammary gland 



Marrow of bone 



Musciilar tissue 



striated . 

 non-striated 



Nerve 

 (Esophagus 

 Ovary 

 Pancreas . 

 Retina 



Salivary glands . 

 Sclerotic and cornea 

 Skin . 



Hardening Fluid 



Chromic acid. 



Alcohol, or bichromate of potash. 



Bichromate of ammonia. 



Bichromate of potash. 



Chromic acid or picric acid. 



Muller's fluid. 



Alcohol. 



Picric acid. 



Alcohol, or bichromate of potash. 



Alcohol. 



Distend with chromic acid. 



Bichromate of potash. 



Alcohol. 



Chromic acid. 



Bichromate of potash. 



Distend with chromic acid. 



Alcohol. 



Alcohol. 



Bichromate of potash. 



Chromic acid. 



Picric acid. 



Distend with chromic acid. 



Chromic acid. 



Alcohol. 



Muller's fluid. 



Alcohol. 



Alcohol, or Muller's fluid. 



Alcohol. 



Methods of preparation required for special purposes are given in the Lessons. 



R 



