CHAPTER V. 53 



Acetic Alcohol with Sublimate. CARNOY and LEBRUN (La 

 Cellule, xiii, 1, 1887, p. 68, due to GILSON). 



Absolute alcohol ...... 1 vol. 



Glacial acetic acid . . . . . 1 ,, 



Chloroform . . . . . . 1 



Sublimate to saturation. 



(The mixture does not keep long, forming ethyl acetate, which 

 precipitates). 



Isolated ova of Ascaris, even though furnished with a shell, are 

 fixed in twenty-five to thirty seconds. Entire oviducts take about 

 ten minutes. The liquid is therefore one of the most penetrating 

 and rapidly acting of any, if not the most. 



Wash out with alcohol until all traces of odour of the acetic acid 

 have disappeared (I myself wash out with alcohol containing 

 tincture of iodine). I consider this a very fine reagent. 

 For Ohlmacher's mixture see 65. 



G. S. SANSOM'S Carnoy Modification. - 



Absolute alcohol . . . . .65 c.c. 

 GUac ; acetic acid . . . . 5 



Chloroform . . . . . 30 ,, 



Corr. subl. to saturation. Leave ten minutes to half an hour ; wash 

 in iodine absolute, then absolute. (Personal communication.) 



Eminently suitable for study of vertebrate material. I have seen 

 some really brilliant results obtained by the use of this fluid ( 590). 



87. Trichlor-acetic Acid (HOLMGREN, Anat. Hefte, xviii, 1901, H. 

 2). Five per cent, solution in water. Fix (nerve-cells) for eight to 

 twenty-four hours, wash out with alcohol. See also HEIDENHAIN, Zeit. 

 iviss. Mikr., xxii, 1905, p. 321, and xxv, 1909, p. 405, who makes a 

 mixture of 6 per cent, sublimate solution with 2 per cent, of trichlor- 

 acetic and 1 per cent, of acetic acid, which he calls " Subtriessig." 



88. Trichlor-acetic Fluid for Batrachia (CHAMP Y, Arch. d. Zool. 

 Exper. et Gen., t. lii, 1913).- 



Carbolic acid cryst. in sat. aq. sol. .- .15 parts. 

 Formol, 40 per cent. . . . 4 ,, 



Trichlor-acetic acid, 20 per cent. . . 1-5 

 Outside of tissue often bad, inner parts better. 



89. Salicylic Acid (HEIDENHAIN, Arch. mik. Anat., liv, 1899, p. 186).- 

 Saturated solution in one-third alcohol. A trial has given me simply 

 atrocious results. 



90. Chloride and Acetate of Copper (Ripart et Petit' s Liquid, 

 CARNOY, La Biologie Cellulaire, p. 94).- 



Camphor water (not saturated) . . 75 grms. 



Distilled water . . . . . 75 



Crystallised acetic acid .... 1 grin. 



Acetate of copper . . . . . O30 



Chloride of copper ..... 0-30 





