156 HMMATEIN (HSEMATOXYLIN) STAINS. 



If the objects stain in too red a tone they may be treated with a 

 solution (of about 2 per cent.) of chloride of aluminium in 70 per 

 cent, alcohol, or with a 0-5 to 1 per cent, solution of acetate of soda 

 or potash in absolute alcohol ; but washing with neutral alcohol 

 will generally suffice. 



With certain objects this solution does not penetrate well. This 

 may be remedied by acidifying the solution, or, which is better, by 

 leaving the objects for some time before staining in acid alcohol. 

 Anyway objects ought NOT to have an alkaline reaction. If these 

 precautions be taken, it will not be necessary to use acid for washing 

 out. 



The solution is not recommended as giving as good results as 

 hsemalum, and Mayer recommends it merely as a substitute for 

 Kleinenberg's, in cases in which an alcoholic haematein stain seems 

 indicated, as being easy to prepare, and constant in its effects. 



262. MAYER'S Hsemastrontium (Grundzuge, LEE and MAYER, 

 1910, p. 166). 1 grm. hsematein, 1 grm. aluminium chloride, 

 50 grms. strontium chloride, 600 c.c. alcohol of 70 per cent., and (if 

 desired) 0-25 grm. citric acid. Prepare and use as hsemacalcium. 



263. DE GROOT'S Alcoholic Hcemalum (Zeit. wiss. Mik., xxix, 1912, 

 p. 182). Mix 20 c.c. of glycerin with 240 of alcohol of 70 per cent. 

 Take 4 c.c. of the mixture, 2 c.c. of hydrogen peroxide, and 0-5 grm. of 

 hsematoxylin, and dissolve with heat. Add 60 c.c. of the mixture, 

 4 grms. of calcium chloride, and 2 grms. of sodium bromide. Dissolve, 

 add 3 grms. of alum, heat and add 100 c.c. of the mixture. When the 

 alum is dissolved add 0-2 grm. of ferri-cyanide of potassium ; dissolve 

 and add 3 grms. more of alum and the rest of the mixture. Said to stain 

 almost as well as hseinalum. Wash out with alcohol of 70 per cent. 



264. Other Alumina-Haematein Solutions. A large number of 

 suppressed receipts will be found given in the earlier editions. 



265. R. HEIDENHAIN'S Chrome Hsematoxylin (Arch. mik. Anat., 

 xxiv, 1884, p. 468, and xxvii, 1886, p. 383). 'Stain for twelve to twenty- 

 four hours in a * per cent, solution of hsematoxylin in distilled water. 

 Soak for the same time in a 0-5 per cent, solution of neutral chromate 

 of potash. Wash out the excess of chromate with water. 



Objects that have been fixed in corrosive sublimate ought to be very 

 carefully washed out with iodine, or the like, as neutral hseinatoxylin 

 forms a black precipitate with any excess of sublimate that may remain 

 in the tissues. See TORNIER, in Arch. mik. Anat., 1886, p. 181. 



The process is adapted to staining in bulk. You can decolour the 

 objects to any extent by prolonging the soaking in the chromate. 

 Bichromate will do instead of the neutral chromate. 



266. APATHY'S Modification of Heidenhain's Process (Zeit. wiss. 

 Mik., v, 1888, p. 47). This is an alcoholic method. Stain in a 1 per 



