CHAPTER XIV. 169 



289. Other Regressive Stains. The following may be useful :- 



Dahlia, according to FLEMMING (Arch. mik. Anat., xix, 1881, 

 p. 317), best used in aqueous solution, either neutral or acidified with 

 acetic acid, and differentiated with neutral alcohol. A pure blue 

 stain, which keeps well. See also SCHUBERG, in Zeit. wiss. ZooL, 

 Ixxiv, 1903, p. 7, and Ixxxvii, 1907, p. 557. 



Victoria Blue (Victoriablau) (LUSTGARTEN, Med. JaJirb. L Ges. d. 

 Aerzte zu Wien, 1886, pp. 285 291). This dye ("Victoriablau 

 4 A ") has a special affinity for elastic fibres. For this object Lust- 

 garten recommends an alcoholic solution of the dye diluted with 

 2 to 4 parts of water. Fixation in chrom-osmium, or at least in a 

 chromic mixture, is, I believe, a necessary condition to this reaction. 

 And you must stain for a long time. 



Victoria has also a special affinity for mucus-cells, from which it 

 is not washed out by alcohol, and for cartilage. 



This stain keeps very well. 



With Toluidin Blue I have had some superb stains of chromatin, 

 unfortunately accompanied by a diffuse staining of cytoplasm. 



MANN (Zeit. wiss. Mik\, xi, 1894, p. 489) states that he has had good 

 results by staining with it after eosin. 



See further, as to the micro -chemical properties of this dye, HARRIS, 

 The Philadelphia Medical Journal, May 14th, 1898. It much resembles 

 methylen blue. 



METZNER (NagePs Handb. Phys., ii, 1907, p. 915) mordants sections, 

 before staining, for three-quarters of an hour in iron alum. 



Magdala Red (Naphthalin Red, Rose de Naphthaline). 



Fuchsin (meaning the basic fuchsins, a series of Eosanilin salts 

 having very similar reactions, and found in commerce under the names 

 of FUCHSIN, ANILIN EED, EUBIN, EOSEIN, MAGENTA, SOLFERINO, 

 CORALLIN). GRASER (Deutsche Zeit. Chirurgie, xxvii, 1888, pp. 538- 

 584 ; Zeit. iviss. Mik., v, 1888, p. 378) stains for twelve to twenty-four 

 hours in a dilute aqueous solution, washes out for a short time in alcohol, 

 stains for a few minutes in aqueous solution of metliyleii blue, and 

 dehydrates with alcohol. A double stain. Chromatin and nucleoli red, 

 all the rest blue. 



ZIEHL'S Carbolic Fuchsin (Zeit. wiss. Mik., vii, 1890, p. 39) consists 

 of fuchsin 1 grm., acid, carbol. crist. 5 grms., alcohol 10 grms., aq. 

 dest. 100 grms. The stain is differentiated with alcohol followed 

 by clove oil. 



Kresofuchsin (Eoxma, Arch. mik. Anat., Ivi, 1900, p. 354). Its 

 aqueous solution is red and stains mucus, cartilage, keratin, and nuclei 

 red, whilst its alcoholic solution is blue and stains elastin blue. See 

 also under " Connective tissues." 



Bismarck Brown has this advantage, that being sufficiently resistant 

 to alcohol it may be utilised for staining entire objects. 



