CHAPTER XV. 183 



Spirit Soluble Blue (Bleu Alcool), Gentian Blue 6 B, Spirit Blue 0, 

 Opal Blue, Bleu de Nuit, Blue Lumiere, Parma Bleu, Bleu de Lyon. 



Some authors give the name Bleu de Nuit and Griindstichblau as 

 synonyms of Bleu de Lyon. The Encycl. mik. Technik. says it is 

 " Anilinblau B- - 6 B," with many synonyms, or designations of 

 brands, Parma blue being " Anilinblau R or 2 R." I find it a fairly 

 good stain, giving very good differentiations of nerve-tissue and of 

 cartilage (as has already been pointed out by BAUMGARTEN and by 

 JACOBY). MAURICE and SCHULGIN stain in bulk with it after borax- 

 carmine, using a very dilute alcoholic solution. BAUMGARTEN and 

 JACOBY stain sections in a 0-2 per cent, alcoholic solution. 



TONKOFF (Arch. mik. Anat., Ivi, 1900, p. 394) adds a little tincture 

 of iodine to the solution of the dye, or mordants the sections with 

 iodine. 



SKROBANSKY (Intern. Monatsschr. Anat., xxi, 1904, p. 20) uses it 

 in water with picric acid. 



327. Carmine Blue (Bleu Carmin Aqueux, from Mesiter, Lucius, and 

 Brunig, at Hochst-a-M.). JANSSENS (La Cellule, ix, 1893, p. 9) states 

 that this colour possesses a special affinity for the parts of cytoplasm 

 that are undergoing cuticular differentiation. He uses it in alcoholic 

 solution acidified. 



328. Methyl Blue. Under this title are comprised some other 

 derivatives of the base rosanilin. They are " acid " colours. Here 

 belong Methyl Blue, Cotton Blue, Water Blue (Wasserblau), Methyl 

 Water-Blue, China Blue (Chinablau), Soluble Blue. 



Amongst these Water Blue (Wasserblau) possesses some useful 

 properties. According to MITROPHANOW (quoted from Zeit. wiss. 

 Mik., v, 1888, p. 513), used in concentrated aqueous solution it 

 gives a very good double stain with safranin. It is very resistant 

 to alcohol. Using the Wasserblau first, and then the safranin, I 

 have had some interesting results. The Wasserblau must be used 

 first. With chrom-osmium material, twelve to twenty-four hours 

 in the blue, and four or five in the safranin, may not be too much. 

 My stains have not kept well. 



MANN (Methods, etc., p. 216) uses a mixture of 35 parts 1 per cent, 

 solution of eosin, 45 of methyl blue 1 per cent., and 100 of water. 

 He has also (Zeit. wiss. Mik., xi, 1894, p. 490) used a similar mixture 

 for nerve-cells. 



329. Anilin Blue-black. A preparation cited under this name has 

 been recommended by BEVAN LEWIS and others for nervous tissue. 

 The dye used by them cannot now be identified. Dr. GRUBLER writes 

 me that the anilin blue-black of his list is the oxyazo colour blue-black B 

 or AZOSCHWARZ ; but that dye had not been discovered when Bevan 



