214 OTHER STAINS AND COMBINATIONS. 



so useful as many others, the eosin lacking in electivity. Objects 

 may be stained with hsematoxylin (either in the mass or as sections) 

 and the sections stained for a few minutes in eosin. I think it is 

 better to take the eosin weak, though it has been recommended 

 (STOHR, see Zeit. wiss. Mik., i, 1884, p. 583) to take it saturated. 

 Either aqueous or alcoholic solutions of eosin may be used. 



HICKSON (Quart. Journ. Mic. Sci., 1893, p. 129) stains sections 

 for one hour in a strong solution of eosin in 90 per cent, alcohol, 

 washes with alcohol, and stains for twenty minutes in a weak solution 

 of hsematoxylin. 



This method is most particularly recommendable for embryo- 

 logical sections, as vitellus takes the eosin stain energetically, and 

 so stands out boldly from the other germinal layers in which the blue 

 of the hsematoxylin dominates. 



See also LIST (Zeit. loiss. Mik., ii, 1885, p. 148) ; BUSCH (Verh. 

 Berl Phys. Gses., 1887) ; GIERKE (Zeit. wiss. Mik., i, 1884, p. 505). 



Sections should be very well washed before being passed from 

 eosin into haematoxylin or the reverse, as eosin very easily precipitates 

 hsematoxylin. 



For the complicated and superfluous mixtures of RENAUT and of 

 EVERARD, DEMOOR and MASSART, see FOL'S Lehrbuch, p. 196, Ann. 

 Inst. Pasteur, vii, 1893, p. 166, or early editions. 



See especially SCOTT'S method, 676. 



393a. Haematoxylin and Azoeosin or Biebrich Scarlet. See under 



" Nucleoli," 676. 



394. Haematoxylin and Congo. See 305. 



395. Haematoxylin and Safranin. RABL (Morph. Jahrb., x, 1884, 

 p. 215) stained very lightly with very dilute DELAFIELD'S hsematoxylin for 

 twenty-four hours, then for some hours in (PFITZNER'S) safranin, and 

 washed out with pure alcohol. The plasma stain is here given by the 

 hsematoxylin. 



Similarly REGAUD, Verh. Anat. Ges., xiv, 1900, p. 112. 



FoA (Festsclir. Virchow, 1891, p. 481) stains in a mixture of 25 c.c. 

 of Bohmer's hsematoxylin, 20 of 1 per cent, solution of safranin, 

 and 100 of water for one to three minutes. 



396. Haematoxylin and Saurefuchsin. Stain first with iron 

 hsematoxylin or hsemalum, then stain (sections) in 0-5 per cent, 

 aqueous solution of Saurefuchsin, dehydrate and mount. 



397. Haematoxylin and Saurefuchsin and Orange. Proceed as 

 above, using for the second stain the following mixture : Saure- 

 fuchsin, 1 grin. ; orange, 6 grms. ; rectified spirit, 60 c.c. ; water, 



