240 INJECTIONS OTHER MASSES (COLD). 



glycerin ; or a saturated alcoholic solution of gamboge added to a 

 mixture of equal parts of glycerin and water. Any excess of alcohol 

 may be got rid of by allowing the mass to stand for twenty-four 

 hours. 



500. Other Colours. Any of the colouring masses, 485 to 498, or 

 other suitable colouring masses, combined with glycerin, either dilute 

 or pure. 



Purely Aqueous Masses. (See 592.) 



501. RANVIER'S Prussian Blue Aqueous Mass (Tmite, p. 120).- 

 The soluble Prussian blue, 494, injected without any vehicle. It 

 does not extravasate. 



502. MULLER'S Berlin Blue (Arch. mik. Anat., 1865, p. 150).- 

 Precipitate a concentrated solution of Berlin blue by means of 

 J to 1 volume of 90 per cent, alcohol. The precipitate is very finely 

 divided ; and the fluid may be injected at once. 



503. MAYER'S Berlin Blue (Mitth. Zool Slat. Neapel, 1888, 

 p. 307). A solution of 10 c.c. of tincture of perchloride of iron in 

 500 c.c. of water is added to a solution of 20 gr. of yellow prussiate 

 of potash in 500 c.c. of water, allowed to stand for twelve hours, 

 decanted, the deposit washed with distilled water on a filter until 

 the washings come through dark blue (one to two days), and the blue 

 dissolved in about a litre of water. It is well to add a little acetic 

 acid and to put up the objects in an acid liquid. 



504. EMERY'S Aqueous Carmine (ibid., 1881, p. 21). To a 10 per cent, 

 ammoniacal solution of carmine is added acetic acid, with continual 

 stirring, until the colour of the solution changes to blood-red. The 

 supernatant clear solution is injected cold without further preparation. 

 The injected organs are thrown at once into strong alcoho] to fix the 

 carmine. For injection of fishes. 



505. TAGUCHI'S Indian Ink (Arch. mik. Anat., 1888, p. 565).- 

 Chinese or (better) Japanese ink well rubbed up on a hone until a 

 fluid is obtained that does not run when dropped on thin blotting- 

 paper, nor form a grey ring round the drop. Inject until the 

 preparation appears quite black, and throw it into some hardening 

 liquid (not pure water). 



DELLA ROSA (Ver. Anat. Ges., 1900, p. 141) recommends the 

 liquid Chinese ink sold in the shops. 



Partially Aqueous Masses. 



506. JOSEPH'S White-of-Egg (Ber. naturw. Sect. Schles. Ges., 

 1879, pp. 3640 ; Journ. Roy. Mic. Soc., ii, 1882, p. 274). " Fil- 



