336 



CYTOLOGICAL METHODS. 



culties arise in the study of oogenesis and gland secretion, where 

 one meets with profound changes in the chromophility of the 

 nucleus, and with complicated nucleoli, whose real nature is as 

 yet undetermined ; moreover, at certain stages in the differentiation 

 of a cell, true chromatin may stain oxyphil. Such exceptions must 

 be taken into consideration ; always ascertain carefully the origin 

 and behaviour of a doubtful body and remember that the safest 

 tests for chromatin lie not in staining, but in the behaviour of the 

 doubtful material during mitosis and in the sexual or reproductive 

 phenomena of the cell. See also GATENBY, Joiim. R. Mic. Soc'., 

 1919 and 1920. 



710. Chromatin and the Inclusions. 



See also remarks on nucleoli in 664 and 668. 



711. Cytoplasmic Inclusions in Gametogenesis. In the table 

 below is a summary of the fixing and staining reactions of the 

 inclusions during oogenesis and spermatogenesis. In the male 

 germ cells the Golgi apparatus (nebenkern batonettes) show through- 



