CHAPTER XXX. 381 



DUBOSCQ (Arch. Zool. Exper., vi, 1899, p. 481) uses (for blood of 

 Chilopoda) a solution of acetic acid, copper acetate, copper chloride, 

 osmic acid, thionin, 1 grm. each, water 400, which, mixed with the 

 blood, fixes and stains in about two minutes. 



Formol has lately been used. MARCANO (Arch, de Med. Exper., 

 xi, 1899, p. 434) mixes fresh blood with a mixture of 100 parts of 

 sodium sulphate of sp. gr. 1-020 and 1 of formol ; or with water 

 85 to 100 parts, sodium chloride 1, and formol 1. 



KIZER (Journ. Roy. Mic. Soc., 1990, p. 128) simply mixes 1 drop 

 of blood with 3 of 2 per cent, formalin, and allows to stand for an 

 hour. 



SCHRIDDE (Hcemat. Techn. Jena, 1910, p. 17) lets blood drop into 

 a mixture of 1 part of formol, 9 of liquid of Miiller, and 10 of water, 

 fixes therein for two to four hours at 40 C., filters, washes and brings 

 through alcohol and chloroform into paraffin for sectioning. 



783. Fixing and Preserving in Films. MUIR (Journ. of Anat. and 

 Phys., xxvi, 1892) makes cover-glass films and drops them into 

 saturated sublimate solution, and after half an hour washes, dehy- 

 drates, and passes through xylol into balsam. 



GULLAND (Br'it. Med. Journ., March 13th, 1897 ; Scottish Med. 

 Journ., April, 1899) makes cover-glass films, and after a few seconds 

 drops them face downwards into a solution of- 



Absolute alcohol saturated with eosin . . 25 c.c. 



Pure ether . . . . . . 25 ,, 



Sublimate in absolute alcohol (2 grms. to 



10 c.c.) ...... 5 drops. 



After three or four minutes they are washed, stained, and mounted 

 in balsam. 



For JENNER'S fixing and staining and staining method, see 

 next section. 



Many recent authors fix wet films with formol. BENARIO (Deut. 

 med. Wochenschr., 1895, p. 572) mixes 1 part of 10 per cent, formol 

 with 9 of 7 . alcohol (the mixture must be freshly prepared), and 

 plunges films into it for a minute. 



Similarly GULLAND, with 1 part of formol to 9 of alcohol. 



Similarly WERMEL (see Zeit. wiss. Mik., xvi, 1899, p. 50), who 

 combines various stains (methylen blue, eosin, gentian, etc.) with 

 the formol. 



EDINGTON (Brit. Med. Journ., 1900, p. 19) exposes films for 

 fifteen to thirty minutes to vapour of formol under a bell-jar. 



SCOTT (Journ. of Path, and Bacter., vii, 1900, p. 131) exposes 



