382 BLOOD AND GLANDS. 



films to the vapour for about five seconds and drops into absolute 

 alcohol, and after fifteen minutes stains and mounts. 



A short exposure (thirty seconds) to vapour of osmium has also 

 been recommended. 



SZECSI (Deutsch. med. Wochschr., 1913, p. 1584) has recommended 

 Lucidol for blood smears, and smears of fseces containing protozoa 

 and cysts. The formulae for an acetone and a pyridin solution will 

 be found on p. 59, 107, and also of an acetone-xylol solution for 

 subsequent washing of the smears. 



It is best to keep a sufficient quantity of the fixing solutions in 

 staining jars. Make a smear, allow it to dry, and place it in the 

 acetone peroxide of benzol solution for fifteen minutes ; transfer 

 to the acetone xylol solution for ten minutes in order to remove the 

 lucidol ; wash off in pure methyl alcohol ; the slide is now ready for 

 staining. It will be found that most of the current stains used for 

 such smears will act successfully after the lucidol fixation. Pappen- 

 heim's panoptic method ( 784) is recommended. 



For smears of fseces a fixation of twenty minutes in the pyridin- 

 benzol peroxide solution is used ; wash as above, in acetone-xylol, 

 or pyridin-xylol, and then in methyl-alcohol. 



Possibly the substitution of pure acetone for the methyl alcohol 

 bath might prove advantageous in some ways. 



784. Stains for Blood. Fresh (unfixed) blood can be stained on 

 the slide. See also 1008, et seq. 



TOISON (Journ. Sci. med. de Lille, fev., 1885 ; Zeit. wiss. Mik., 

 1885, p. 398) recommends that it be mixed with the following 

 fluid : 



Distilled water . . . . 160 c.c. 

 Glycerin (neutral, 30 Baume) . 30 



Pure sulphate of sodium ... 8 grammes. 

 Pure chloride of sodium ... 1 gramme. 

 Methyl violet 5 B . . . . 0-5 

 (The methyl violet is to be dissolved in the glycerin with one half 

 of the water added to it ; the two salts are to be dissolved in the 

 other half of the water, and the two solutions are to be mixed and 

 filtered.) This mixture stains leucocytes sharply, which facilitates 

 enumeration. 



BIZZOZERO and TORRE (Arch. Sci. Mediche, 1880, p. 390) dilute 

 a drop with normal salt solution containing a little methyl violet, 

 which stains nuclei intensely, cytoplasm less intensely. 



Similarly GiGLio-Tos (Zeit. wiss. Mik., 1898, p. 166), diluting 

 with saturated solution of neutral red in salt solution, which stains 



