CHAPTER XXXII. 441 



his reduced silver and uranium nitrate methods, for which see Trab. 

 Lab. Invest. Biol., Madrid, x, 1912, p. 221. 



851. Methods demonstrating Neurokeratin Network. PLATNER 

 (Ztschr. wiss. Mikr., vi, 1889, p. 186) fixes for several days in a 

 mixture of 1 part of Liq. Ferri Perchlor. (Ph. G., ed. 2) and 3 to 4 

 parts of water or alcohol, washes out well in water and stains for 

 several days or weeks in a concentrated solution of " Echtgriin' 

 (dinitroresorcin) in 75 per cent, alcohol. See also BEER, Jahrb. 

 Psychiatric, ii, 1893. 



Cox (Aiiot. Hefte, i, 1898, p. 102, note) fixes nerves in 2 per cent, 

 osmic acid (rabbit) or 1 per cent, (frog), washes, dehydrates, clears 

 with bergamot oil, and mounts in balsam. The bergamot oil dissolves 

 out the myelin, and leaves the neurokeratin visible. It may be 

 necessary to leave the nerves for forty-eight hours in the oil. 



CORNING (Anat. Am., xvii, 1900, p. 309) studies the neurokeratin 

 network in the sciatic of the frog by means of sections of sublimate 

 material strongly stained with iron ha3matoxylin. 



KAPLAN (Arch. Psychiatr., xxxv, 1902, p. 825) stains sections with 

 acid fuchsin and differentiates them by Pal's method. 



GEDOELST (La Cellule, v, 1889, p. 136) has the following : (a) A 

 nerve is treated with liquid of Perenyi, either pure or with addition 

 of a trace of osmic acid, and examined in glycerin. By this treat- 

 ment the myelin loses its excessive refractivity and the neurokeratin 

 network comes out clearly. (6) Silver nitrate. Good images, but 

 uncertain, (c) Treatment with a mixture of 1 per cent, osmic 

 acid and absolute alcohol. The network comes out black. 



