CHAPTER XXXIII. 447 



858. KAISER (Neurol. GentrbL, xii, 1893, pp. 364) hardens first in 

 Miiller's fluid, then for eight days in Marchi's fluid ( 870), mordants 

 sections for five minutes with sesquichloride of iron (1 part to one 

 of water and 3 of 70 per cent, alcohol), stains and differentiates with 

 Pal's liquid. For details see early editions. 



BOLTON (Journ. Anat. & Phys., xxxii, 1898, p. 247) makes sections 

 of formalin material, and mordants them for a few minutes in 1 per cent, 

 osmic acid, or for a few hours in iron-alum or ammonium molybdate, 

 stains in KULTSCHITZKY'S haematoxylin (next ), and differentiates by 

 Pal's process. 



Similarly WYNN, ibid., xxxiv, 1900, p. 381. 



LASLETT (Lancet, 1898, p. 321) mordants in Marchi's fluid (1 week), 

 makes sections, stains by KULTSCHITZKY'S method, and differentiates 

 by PAL'S. 



859. KULTSCHITZKY'S Method (Anat. Anz., iv, 1889, p. 223 ; and v, 

 1890, p. 519). Specimens are hardened for one or two months in 

 ERLICKI'S fluid, imbedded in celloidin or photoxylin, and cut. 

 Sections are stained for from one to three hours, or as much as 

 twenty-four, in a stain made by adding 1 grm. of hsematoxylin 

 dissolved in a little alcohol to 100 c.c. of 2 per cent, acetic acid. 

 They are washed out in saturated solution of lithium or sodium 

 carbonate. Differentiation is not necessary, but by adding to the 

 lithium carbonate solution 10 per cent, of a 1 per cent, solution of 

 potassium red prussiate, and decolorising therein for two or three 

 hours or more, a sharper stain is obtained. After this the sections 

 are well washed in water and mounted in balsam. Myelin dark blue. 



WOLTERS (Ztschr. wiss. Mikr., vii, 1890, p. 466) proceeds as 

 Kultschitzky, except that he stains at 45 C. for twenty-four hours, 

 after which the sections are dipped in Miiller's fluid, and 

 differentiated by Pal's method. 



Similarly KAES (Neurol. CentrbL, x, 1891, p. 456). Myelin dark 

 blue, cells yellow-brown. 



860. MITROPHANOW (Ztschr. wiss. Mikr., xiii, 1896, p. 470) mordants 

 photoxylin sections for at least twenty-four hours at 40 0. in a mixture 

 of equal parts of saturated aqueous solution of copper acetate and 90 

 per cent, alcohol, stains for ten minutes in KULTSCHITZKY'S hsernatoxy- 

 lin, and differentiates with Weigert's ferricyanide fluid. 



861. BERKLEY'S Rapid Method (Neurol. CentrbL, xi, 1892, p. 270). 

 -Slices of tissue of not more than 2J mm. in thickness are 



hardened for twenty-four to thirty hours in FLEMMING'S fluid, at 

 a temperature of 25 C., then in absolute alcohol, then imbedded in 

 celloidin and cut. After washing in water the sections are put 

 overnight into a saturated solution of copper acetate (or simply 

 warmed therein to 35 to 40 C. for half an hour). They are then 



