CHAPTER XXXVI. 501 



by subcutaneous injection of about 1 c.c. of a 5 to 10 per cent, 

 solution of hydrochlorate of cocaine (ROBERT, Bull. Sclent, de la 

 France, etc., 1890, p. 449 ; Zeit. iviss. mik., ix, 1892, p. 216), or 

 (SCHONLEIN, Zeit. BioL, xxx, 1893, p. 187) 1 c.c. of 4 per cent, 

 solution of Pelletierin. For Lo BIANCO 's various methods see the 

 original, p. 467. 



For Pteropoda in general, liquid of Perenyi. Creseis is a difficult 

 form. Lo BIANCO advises the alcohol method, 18. For the 

 Gymnosomata he narcotises with 0-1 per cent, chloral hydrate. 



For terrestrial Gastropods see 23 and 26. MARCHI (Arch. mik. 

 Anat., 1867, p. 204) gets rid of the mucus of the integument of 

 Limax, which may be an obstacle to preparation, by putting the 

 living animal into moderately concentrated salt solution, in which 

 it throws off its mucus and dies in a few hours. 



LANG (Anat. Hefte, 1902, p. 84) puts Helix into water with enough 

 chromic acid to make it of a Rhine-wine colour, with an air-tight 

 cover to the vessel, and when the animals are extruded injects into 

 them a quarter to a half of a Pravaz syringe of 1 per cent, cocaine, 

 and after five to fifteen minutes dissects and fixes. 



HEYMANS (Bull. Acad. Belg., xxxii, 1896, p. 578) injects ethyl 

 bromide under the skin of Cephalopoda. 



Lo BIANCO uses for fixing them his chromo-acetic acid, No. 1 

 ( 39), with a double quantity of acetic acid, for twenty-four hours. 



933. Liver of Mollusca. ENRIQUES (Mitth. Zool. Stat. Neapel, 

 xv, 1901, p. 289) fixes the liver of Octopus and Sepia with sublimate. 

 For Aplysia (especially in summer) alcohol, formol, and chromic 

 mixtures are counter-indicated, on account of the carbohydrates in 

 the cell. Sublimate is best. 



934. Nervous System of Pulmonata. B. de NABIAS (Act. Soc. 

 Linn. Bordeaux, 1894 ; Rech. Hist, centres nerveux des Gasteropodes, 

 1894, p. 23) opens the animals and fixes the ganglia for one hour 

 in a mixture of 6 parts glacial acetic acid to 100 of 90 per cent, 

 alcohol, or for fifteen to twenty minutes in 5 per cent, sublimate 

 with 5 per cent, acetic acid. He stains in bulk, with Renaut's 

 hsematoxylic eosin, or R. Heidenhain's hsematoxylin, or a copper 

 hsematoxylin of Viallanes, and embeds in paraffin. He also stains 

 by the rapid method of GOLGI, embedding, however, the ganglia 

 in celloidin directly after the hardening in osmic acid and bichromate, 

 and treating the sections with the silver (p. 458). He stains with 

 methylen blue by treating the ganglia in situ for twelve to twenty- 

 four hours with a 1 per cent, solution. 



