CHAPTER XXXVI. 509 



HENNJNGS (Zeit. wiss. Mik., xvii, 1900, p. 326) depigments 

 sections by putting them for ten minutes (Musca) to twelve hours 

 (Myriopoda) into a mixture of 2 parts of 80 per cent, alcohol with 1 

 of glycerin and 2 per cent, of nitric acid, best kept at 35 C. The 

 elements are well preserved. 



WIDMANN (Zeit. wiss. ZooL, xc, 1908, p. 260) makes the lens of 

 Arachnida fit for sectioning by putting for a day or so into alcohol 

 with 10 to 15 per cent, of nitric acid ; and bleaches sections with 

 1 part of chlorine water to 2 of alcohol. 



See also ROSENSTADT, Arch. mik. Anat., xlvii, 1896, p. 478 ; 

 VIALLANES, Ann. Sci. Nat., xiii, 1892, p. 354 ; and DIETRICH, Zeit. 

 wiss. ZooL, xcii, 1909, p. 465 (fixes in alcoholic formol, and bleaches 

 with dilute aqua regia). 



952. Injections (Arachnida and Crustacea especially). AIME 

 SCHNEIDER (Tablettes Zool., ii, 1892, p. 123) recommends lithographic 

 Indian ink, the animals being narcotised with chloroform, then 

 injected and thrown into strong alcohol. Similarly CAUSARD (Bull. 

 8c. France Belg., xxix, 1896, p. 16). 



953. Aretiseoida (DOYERE, Arch. mik. Anat., 1865, p. 105). Examina- 

 tion of living animals after partial asphyxiation in boiled water. See 

 early editions. 



Vermes. 



954. Chsetopoda : Cleansing Intestine. KUKENTHAL (Journ. 

 Roy. Mic. Soc., 1888, p. 1044) puts Lumbricus into a glass vessel 

 filled with bits of moistened blotting-paper. They gradually 

 evacuate the earthy particles from the gut, and fill it instead with 

 paper. 



VOGT and YUNG (Traite d'Anat. Comp. Prat., v) recommend 

 coffee-grounds instead of paper, as they cut better after embedding. 



JOEST (Arch. Entwicklungsmech., v, 1897, p. 425) simply keeps 

 the worms for a few days in moist linen, and finds the gut empty. 



PEARL (Journ. appl. Mic., iii, 1901, p. 680) injects alcohol of 6 per 

 cent, through the gut of narcotised worms. 



955. Chsetopoda : Fixation. Lumbricus may be anaesthetised 

 by putting the animals into water with a few drops of chloroform. 

 PERRIER puts them into water in a shallow dish, sets up a 

 watch-glass with chloroform in the corner of it, and covers the 

 whole. 



CERFONTAINE (Arch, de Biol., x, 1890, p. 327) injects interstitially 

 about 2 c.c. of a 1 : 500 solution of curare. 



