542 METHODS FOR INVERTEBRATES. 



Soc., 1885, p. 538) fixes for a few minutes with J per cent, solution 

 of chloride of palladium. 



BRASS (Zeit. iviss. Mik., 1884, p. 39) employs a mixture of 1 part 

 each of chromic acid, platinum chloride, and acetic acid with 400 to 

 1,000 parts of water. 



CERTES (Comptes rend., Ixxxviii, 1879, p. 433) fixes with 2 per cent, 

 osmic acid, or its vapours (ten to thirty minutes). For details, see 

 previous editions. 



LONGHI (Bull. Mm. Zool. Univ. Genova, 1892, No. 4) kills in 

 10 c.c. of 1 per cent, sulphate of eserin with 1 drop of 1 per cent, 

 sublimate. 



SCALA (Rev. Mus. La Plata, xv, 1908, p. 57) fixes for five or ten 

 minutes in a mixture of 2 mg. of atropin, 10 drops of formol, 10 grms. 

 of glycerin and 50 c.c. of water. 



See also PUSCHKAREW, Zeit. wiss. Mik., xxviii, 1911, p. 145 (agar 

 process for fixing and staining Amoebae). 



FOL (Lehrb., p. 102) fixes delicate marine Infusoria (Tintinnodea) 

 with the perchloride of iron solution ( 80), added to the water 

 containing them, and stains with gallic acid. 



Lo BIANCO (loc. cit., p. 444) fixes Gregarinee with picro-sulphuric 

 acid (one hour), Vorticellae with hot sublimate, Acinetse with subli- 

 mate in sea water, or with osmic acid, Thalassicola with 0-5 per cent, 

 chromic acid (one hour), Acanthometrse and Aulacanthae with 50 per 

 cent, alcohol or with concentrated sublimate, or by adding a little 

 osmic acid to the water. For Sphaarozoa he proceeds as BRANDT, 

 1019. 



ZOGRAF fixes Rhizopoda and Infusoria as Rotatoria, 886, but 

 without narcotisation. 



See also FABRE-DOMERGUE, Ann. de Microgr., ii, 1889, p. 545, and 

 1890, p. 50 ; SCHEWIAKOFF, Biblioth. Zool., v, 1889, p. 5 ; Journ. Roy. 

 Mic. Soc., 1889, pp. 832, 833 ; ZOJA, Boll. Sci. Pavia, 1892 ; Zeit. wiss. 

 Mik., ix, 1893, p. 485 ; LAUTERBORN, Zeit. wiss. Zool., lix, 1895, p. 170 ; 

 SCHAUDINN, ibid., p. 193; BALBIANI, Zool. Ans., xiii, 1890, p. 133; 

 KARAWAIEW, ibid., xviii, 1895, p. 286. 



1022. Embedding of Protozoa and other Small Objects (MINCHIN, 

 Q. J. M. S., Ix, 1915, p. 508). A thin slice of a block of amyloid 

 liver preserved in alcohol is floated into a shallow glass vessel with 

 a flat bottom, containing alcohol. The dish is placed on the stage 

 of a dissecting microscope. The objects to be embedded are taken 

 up in a pipette and placed on the slice of liver and orientated as 

 desired. 



A tiny drop of glycerine and albumen solution is taken up on the 



