CHAPTER V. 47 



mends a solution of 3 to 4 grms. of sublimate and 0-5 grm. 

 sodium chloride in 100 c.c. of 50 per cent, alcohol for general pur- 

 poses. 



OHLMACHER (Journ. Ejrper. Medicine, ii, 6, 1897, p. 671) takes- 

 Absolute alcohol . . . . .80 parts. 

 Chloroform . . . . . 15 

 Glacial acetic acid . . . . 5 ,, 

 Sublimate to saturation (about 20 per cent.). 



" Ordinary pieces " of tissue are sufficiently fixed in fifteen to thirty 

 minutes. Entire human cerebral hemispheres, subdivided by Meynert's 

 section, take eighteen to twenty-four hours. 



For liquids containing a much higher proportion of acetic acid, see 

 Acetic Alcohol. 



* 



66. Acetone Solution. HELD (Arch. Anat. Phys., Anat. Abth., 

 1897, p. 227) fixes nerve-tissue in a 1 per cent, solution of sublimate 

 in 40 per cent, acetone, and washes out through increasingly con- 

 centrated grades of acetone. 



67. Phenol Solution. PAPPENHEIM (Arch. Path. Anat., clvii, 1899, 

 p. 23) shakes up carbolic acid with aqueous sublimate solution and 

 filters. 



68. Ciaccio (Arch. Ital. Anat. Embr., vi, 1907, p. 486) has an irrational 

 mixture of sublimate, iodine, and formol. 



69. Mercuro-nitric Mixtures. FRENZEL (Arch. mik. Anat., xxvi, 

 1885, p. 232) recommends a half-saturated solution of sublimate in 

 80 per cent, alcohol, to which is added nitric acid in the proportion 

 of 1 drop to 1 or 2 c.c. Objects of the size of a pea to be fixed 

 in it for five or ten minutes, then hardened in the same sublimate 

 alcohol without the acid, and finally in 90 per cent, alcohol. It is 

 said that the nitric acid renders after-treatment with iodine un- 

 necessary. 



GILSON'S Mixture (GILSON, in litt. 1895). 

 Nitric acid of 46 strength (this 

 would be sp. gr. 1-456, or 80 per 

 cent., nearly) .... 15 c.c. 

 Glacial acetic acid . . . . 4 

 Corrosive sublimate ... 20 grms. 



60 per cent, alcohol . . . 100 c.c. 



Distilled water .... 880 ,, 

 When required for marine animals add a few crystals of iodine, 

 which will prevent the formation of precipitates of sea salts. If in 

 any case the preparations should show a granular precipitate, this 

 may be removed by washing with water containing a little tincture 

 of iodine. 



