48 FIXING AND HARDENING AGENTS. 



I find that it affords in general a faithful and delicate fixation, 

 and gives to tissues an excellent consistency. Objects may remain 

 in it for a considerable time without hurt. It has a high degree of 

 penetration. A treatment for a few days with it will serve to 

 remove the albumen from the ova of Batrachians. This liquid may 

 be recommended to beginners, as it is very easy to work with. For 

 some objects, as I found, the proportion of sublimate may be in- 

 creased with advantage. 



KOSTANECKI and. SIEDLECKI (Arch. mik. Anat., xlviii, 1896, p. 181) 

 take a mixture of saturated sublimate solution and 3 per cent, nitric 

 acid in equal parts, or a mixture of equal parts of sublimate solution, 

 3 per cent, nitric acid, and absolute alcohol, fix for twenty-four 

 hours, and wash out in iodine-alcohol. 



PETRUNKEWITSCH (Zool. Jahrb. Abth. Morpk., xiv, 1901, p. 576) 

 takes water 300, absolute alcohol 200, glacial acetic acid 90, nitric 

 acid 10, and sublimate to saturation. Both this and Grilson's have 

 been much used lately. 



70. Picro-sublimate Mixtures. EABL'S (Zeit. wiss. Mik., xi, 1894, 

 p. 165). Sublimate, saturated solution in water, 1 vol. ; a similar 

 solution of picric acid, 1 vol. ; distilled water, 2 vols. Embryos 

 may be left in it for twelve hours, washed for two hours in water, 

 and brought into weak alcohol. 



0. vom BATH (Anat. Anz., xi, 1895, p. 268) takes cold saturated 

 solution of picric acid, 1 part ; hot saturated solution of sublimate, 

 1 part ; glacial acetic acid, -J to 1 per cent. Also the same with the 

 addition of 10 per cent, of 2 per cent, osmic acid solution. 



71. Osmio-sublimate Mixtures. MANN'S (Zeit. wiss. Mik., xi, 

 1894, p. 481) consists of a freshly prepared mixture of equal parts 

 of 1 per cent, osmic acid solution and saturated solution of sublimate 

 in normal salt solution (for nerve-centres). See " Mann-Kopsch 

 Method," 693. 



72. Chromo-sublimate. Lo BIANCO (Mitth. Zool. Stat. Neapel, 

 ix, 3, 1890, p. 443). Concentrated sublimate solution, 100 parts ; 

 1 per cent, chromic acid, 50 parts. 



MANN (Verh. Anat. Ges., 12, 1898, p. 39) takes for nerve-cells equal 

 parts of 5 per cent, sublimate and 5 per cent, chromic acid. 



73. Sublimate and Bichromate. ZENKER'S Mixture (Miinchener 

 med. Wochenschr., xxiv., 1894, p. 534 ; quoted from MERCIER, Zeit. 

 wiss. MiL, xi, 4, 1894, p. 471). Five per cent, of sublimate and 

 5 per cent, of glacial acetic acid dissolved in solution of MULLER. 



