CHAPTER VII. 75 



taining molten paraffin. After this has been solidified by cooling 

 (see next chapter), a block containing the object is cut out of it, and 

 mounted on the object-holder of the microtome (this is, of course, 

 applicable to other masses, such as celloidin). 



For imbedding very small objects in this way certain precautions may 

 be necessary in order not to lose them. SAMTER (Zeit. wiss. Mik., xi, 

 1894, p. 469) saturates small unstained objects with paraffin that has 

 previously been strongly coloured with alkanna extract, and then 

 imbeds them in pure paraffin. EIIUMBLER (ibid., xii, 1895, p. 312, and 

 xiii, 1896, p. 303) stains previously the objects themselves with eosiii 

 dissolved in strong alcohol, and removes the stain from the sections with 

 weak alcohol. See also ibid., xiii, p. 200, a paper by SCHYDLOWSKI ; 

 and in Zeit. wiss. Zool., Iviii, 1897, p. 144, a process of BORGERT. 



BORGERT (Zeit. wiss. Zool., Iviii, 1897, p. 144) allows paraffin to 

 solidify in a watch-glass, bores a hole in it, and places the objects in the 

 hole with a little benzol, and puts the whole for a short time into a stove. 



A watch-glass provided at the bottom with a groove or trough, in 

 which small objects may be made to collect, is described by LEFEVRE, 

 Journ. App. Mic., v, 1902, p. 280 (see Journ. Eoy. Mic. Soc., 1903, 

 p. 233). 



LAUTERBORN (Zeit. iviss. Zool., lix, 1895, p. 170) brings the objects 

 through chloroform into paraffin in a small glass tube, and after cooling- 

 breaks the tube and so obtains a cylinder of paraffin with the objects 

 ready for cutting. 



HOYER (Arch. mik. Ancit., liv, 1899, p. 98) performs all the operations 

 in a glass cylinder (5 centimetres long and 7 millimetres wide), open at 

 both ends, but having a piece of moist parchment paper tied over one 

 of the openings. It is then not necessary to break the cylinder ; by 

 removing the parchment paper the paraffin can be pushed out of it 

 in the shape of a cylinder containing the objects imbedded at one end 

 of it, 



MAYER (Zeit. wiss. Mikr., xxiv, 1907, p. 130) takes the gelatin capsules 

 used by chemists ; after cooling in water the gelatin swells and is easily 

 removed. 



MEVES (Arch. mikr. Anat., Ixxx, Abth. ii, 1912, p. 85) employs wedge- 

 shaped capsules made by G. Pohl, Schonbaum, Bez, Dantzig. 



