158 HMMATEIN (HSEMATOXYLIN) STAINS. 



See also RIBBERT (Centralb. allg. Path., vii, 1896, p. 427 ; Zeit. wiss. 

 Mik., xv, 1898, p. 93), PATELLANI (Mon. Zool. Ital., xiii, 1902, p. 6), and 

 GOLOVIN (Zeit. wiss. Mik., xix, 1902, p. 184). 



SARGENT (Anat. Anz., xv, 1898, p. 214) quotes this stain, preceded by 

 mordanting for twenty -four hours in 5 per cent, sulphate of copper, as 

 KENYON'S. 



KODIS (Arch. mik. Anat., lix, 1901, p. 211) takes hsematoxylin, 1 part ; 

 molybdic anhydride, 1-5 ; water, 100 ; H 2 2 , 0-5, or a crystal of HgO. 



POLICE (Arch. Zool. Napoli, iv, 1909, p. 300) takes 0-35 grm. hsema- 

 toxylin, 10 drops phospho -molybdic acid of 10 per cent., 10 grms. chloral 

 hydrate, and 100 grms. alcohol of 70 per cent. 



272. MALLORY'S Phospho-tungstic Hsematoxylin (Journ. Exj). 

 Med-., v, 1900, p. 19 ; Zeit. wiss. Mik., xviii, 1901, p. 178) : 



Hsematoxylin . . . . . .0-1 



Water ....... 80-0 



10 per cent, solution of (MERCK'S) phospho- 

 tungstic acid . . . . . .20-0 



Peroxide of hydrogen (U.S. Ph.) ... 0-2 

 (Dissolve the hsematoxylin, add the acid, then the peroxide.) Stain 

 sections two to twenty-four hours, wash out with water. A poly- 

 chromic stain, nuclei blue, intercellular substances pink. I consider 

 this a fine stain. 



273. DONAGGIO'S Tin Haematoxylin (Ann. Nevrol. Napoli, xxii, 1904, 

 p. 192). A 1 per cent, solution of hsematoxylin is poured slowly into 

 an equal volume of 20 per cent, solution of pink-salt (ammonio-chloride 

 of tin). Keep in the dark. 



274. Osmium Haematoxylin. SCHULTZE (Zeit. wiss. Mik., xxvii, 

 1910, p. 465) treats tissues for twenty-four hours or more with osmic 

 acid of 1 per cent., washes well with water, and puts for a couple of 

 days into ripened 0-5 per cent, solution of hsematoxylin in alcohol of 35 

 to 50 per cent. Wash out for a day or more with alcohol of 70 per cent. 

 Intense plasma stain. 



