CHAPTER XV. 181 



312. Methyl Green and Eosin (CALBERLA, Morph. Jahrb., iii, 1877, 

 Heft 3, p. 625 ; LIST, Zeit. iviss. Mik., ii, 1885, p. 147 ; BALBIANI, Ann. 

 Microgr., Paris, vii, 1895, p. 245 ; RHUMBLER, Zeit. wiss. Zool., Ixi, 1895, 

 p. 38). See early editions. 



313. Methylen Blue and Eosin (CIIENZINSKY, quoted from Zeit. wiss. 

 Mik., xi, 2, 1894, p. 269). 



Methylen blue, sol. sat. in water . . .40 

 Eosin, 0-5 per cent, in 70 per cent, alcohol . 20 

 Distilled water, or glycerin .... 40 

 This solution will only keep for about eight days. 

 PIANESE (ibid., xi, 1894, p. 345) adds a considerable proportion of 

 carbonate of lithia. 



See also the mixture of BREMER (Arch. mik. Anat., xlv, 1895, p. 446). 

 I have tried CHENZINSKY'S mixture as a tissue stain, without good 

 results ; but see ROSIN, Berliner klin. Wochenschr., 1898, p. 251 ; ^Zeit. 

 wiss. Mik., xvi, 1899, p. 223, and xvii, 1900, p. 333. 



See also LAURENT (Centralb. allg. Path., xi, 1900, p. 86 ; Zeit. wiss. 

 Mik., xvii, 1900, p. 201). 



314. MALLORY'S Eosin and Methylen Blue (Journ. Med. Research, 

 January, 1904). Sections of ZENKER material (other sublimate material, 

 not so good) are stained for half to three quarters of an hour at 56 C. 

 in 5 per cent, aqueous solution of eosin, rinsed and flooded with solution 

 of 1 part of methylen blue, and 1 of potassium carbonate in 100 of 

 water, diluted with about 7 parts of water. After forty minutes they 

 are flooded (not washed) with water, and differentiated for about five 

 minutes in alcohol of 95 per cent. Absolute alcohol, xylol, balsam. 



315. Other Eosin and Methylen-blue Stains. For some very 

 important ones see under " Blood." 



316. Light Green (Lichtgriin S. F.). An " acid' colour, soluble 

 in alcohol, and a good plasma stain. 



BENDA (Verh. physiol. Ges. Berlin, December 18th, 1891, Nos. 

 4 u. 5) stains sections for twenty-four hours in anilin-water safranin 

 solution, then for about half a minute in a solution of 0-5 grm. 

 Lichtgriin or Saureviolett (Grubler) in 200 c.c. of alcohol, dehydrates 

 and mounts in balsam. This process gives a very elegant stain, but 

 requires very thin sections, and there is always risk of the safranin 

 being washed out. The Lichtgriin stain unfortunately does not 

 keep at all well. 



See also PRENANT, Arch. mik. Anat., vii, 1905, p. 430, and 

 GUIEYSSE, C.R. Soc. Biol, Ixii, 1907, p. 1212. 



317. Janus Green (MICHAELIS, Arch. mik. Anat., Iv, 1900, p. 565).- 

 Used in solution of 1 : 30,000 for staining certain granules (pancreas, 

 salivary glands, etc.) in the fresh state. 



318. Malachite Green (syn. Solid Green, Victoria Green, New Green, 

 Benzoyl Green, Fast Green). A basic colour, which has been used as a 



