CHAPTER XVIII. 213 



de Cienc. med., 1895 ; CALLEJA, Rev. trim. Microgr., ii, 1897, p. 101 ; 

 Zeit. wiss. Mik., xv, 1899, p. 323). For use after a carmine stain, 

 RAMON takes a solution of 0-25 grm. of indigo- carmine in 100 grms. 

 saturated aqueous solution of picric acid. Stain (sections) for five 

 to ten minutes, wash in weak acetic acid, then in water, then remove 

 the excess of picric acid with absolute alcohol, clear and mount. 



RAMON also (Elementos de Histologia, 1897 ; quoted from La 

 Cellule, xix, 1901, p. 212) employs the picro-indigo mixture after 

 Magenta ; stain strongly in saturated solution of magenta, rinse in 

 water until no more colour comes away, and pass into the indigo 

 mixture. See also BORREL, Ann. Inst. Pasteur, 1901, p. 57, or 

 LEE et HENNEGUY, Traite, p. 268. 



388. Carmine and Anilin Blue (or Bleu Lumiere, or Bleu de Lyon) 



(DuvAL, Precis de Technique Microscopique, 1878, p. 225). Stain 

 with carmine ; dehydrate, and stain for a few minutes (ten minutes 

 for a section of nerve-centres) in a solution of 10 drops of saturated 

 solution of anilin blue in alcohol to 10 grms. of absolute alcohol. 

 Clear with turpentine, without further treatment with alcohol, and 

 mount in balsam. 



Other authors recommend, instead of anilin blue, bleu de Lyon, 

 dissolved in 70 per cent, alcohol acidulated with acetic acid (MAURICE 

 and SCHULGIN), or bleu lumiere. 



The solutions of both these colours should be extremely dilute for 

 sublimate material, but strong for chrom-osmium material. It is 

 possible to use them for staining in bulk. 



BAUMGARTEN (Arch. mik. Anat., xl, 1892, p. 512) stains sections 

 (of material previously stained in borax-carmine) for twelve hours 

 in a 0-2 per cent, solution of bleu de Lyon in absolute alcohol, and 

 washes out for about half that time before mounting in balsam. 

 He recommends the process for cartilage and nerve-centres. 



389. Carmine and Malachite Green. MAAS (Zeit. wiss. Zool., 1, 4, 

 1890, p. 527) recommends borax-carmine followed by weak alcoholic 

 solution of malachite green, with a final washing out with stronger 

 alcohol. 



390. Carmine and Picro-nigrosin (PIANESE). See Journ. Boy. Mic. 

 Soc., 1892, p. 292. 



391. Carmine and Picric Acid. See 298. 



Hcematein or Hcematoxylin Combinations. 



392. Hsematoxylin and Picric Acid. See 298. 



393. Haematoxylin and Eosin. This popular combination gives 

 results that are aesthetically beautiful, but (for most objects) is not 



