CHAPTER XXII. 241 



tered white-of-egg, diluted with 1 to 5 per cent, of carmine solution. 

 . . . This mass remains liquid when cold, coagulates in dilute nitric 

 acid, chromic or osmic acid, and remains transparent in the vessels." 

 For invertebrates. 



GROSSER (Zeit. wiss. Mik., xvii, 1900, p. 178) rubs up Indian ink 

 with white-of-egg ; HOFFMANN (Zeit. Morph. Anthrop., iii, 1901, 

 p. 240) with blood-serum ; so also HAMBURGER, Zeit. wiss. Mik., 

 xxv, 1908, p. 1 (2 vols. of the ink " Perltusche " to 3 of serum). 



507. BJELOUSSOW'S Gum Arabic Mass (Arch. Anat. Phys., 1885, 

 p. 379). Make a syrupy solution of gum arabic and a saturated 

 solution of borax in water. Mix the solutions in such proportions 

 as to have in the mixture 1 part of borax to 2 of gum arabic. Rub 

 up the transparent, almost insoluble mass with distilled water, 

 added little by little, then force it through a fine-grained cloth. 

 Repeat these operations until there is obtained a mass that is free 

 from clots. It should then coagulate in the presence of alcohol, 

 undergoing at the same time a dilatation to twice its original volume. 

 The vehicle thus prepared may be combined with any colouring 

 mass except cadmium and cobalt. 



After injection the preparation is thrown into alcohol, and the 

 mass sets immediately, swelling up as above described, and conse- 

 quently showing vessels largely distended. 



Cold-blooded animals may be injected whilst alive with this 

 mass. It does not flow out of cut vessels. Injections keep well in 

 alcohol. If it be desired to remove the mass from any part of a 

 preparation, this is easily done with dilute acetic acid. 



508. Milk has been recently recommended by FISCHER (Centralb. 

 ally. Path., xiii, 1902, p. 277 ; Zeit. wiss. Mik., xx, 1903, p. 224). 

 It runs well, does not extra vasate, and can be used for auto-injection 

 of the living subject. 



After injection it should be coagulated by putting the organs 

 for at least twenty-four hours into a mixture of 75 parts of formol ; 

 15 of acetic acid, and 1,000 of water (pure formol will not do). They 

 are then sectioned, and the sections stained with Sudan III or 

 Scharlach R, which stain the milk. They cannot be mounted in 

 balsam. 



Celloidin and other Masses. 



509. SCHIEFFERDECKER'S Celloidin Masses (Arch. Anat. Phys., 1882 

 [Anat. Abth.], p. 201). (For Corrosion preparations.) See previous 

 editions; HOCHSTETTER'S Modification of SCHIEFFERDECKER'S Mass 

 (Anat. Anz., 1886, p. 51) ; BUDGE'S Asphaltum Mass (Arch. mil'. Anat., 



M. 16 



