322 



CYTOLOGICAL METHODS. 



flat-worms, for instance, prepared by Champy-Kull show beautiful 

 colour graduations in their different tissues. The preparations 

 begin to fade after a year. 



After Champy-Kull fixation you can : (a) stain in iron hsema- 

 toxylin (long method, 242), (6) stain as for Benda ( 683), (c) mount 

 unstained for examination of osmicated granulations, (d) stain in 

 safranin and light green ( 286). For a chart illustrating Champy- 

 Kull technique, see below. 



MAXIMOW (C. E. Soc. Biol., Paris, Ixxix, p. 462) fixes in Champy, 

 washes slightly in water, transfers to mixture of 1 per cent, chromic acid 

 i part, glacial acetic 2 parts, for twenty -four hours. Wash again for 

 half -an -hour, place for three days in 3 per cent. K 2 Cr. 2 7 . Wash in 

 running water. Stain sections as above. 



682. Champy-Kull Fixation. 



683. BENDA'S Alizarin Method (Ergebnisse der Anat., xii, 1902 

 (1903), p. 752, and other places) is as follows : Harden for eight 

 days in strong liquid of Flemming, the acetic acid therein being 

 reduced to 3 drops (or as for Champy-Kull or Regaud). Wash for 

 an hour in water and put for twenty-four hours into a mixture of 

 equal parts of pyroligneous acid and 1 per cent, chromic acid, then 

 for twenty-four hours into bichromate of potash of 2 per cent., wash 

 for twenty-four hours and embed in paraffin. Sections on the slide 

 are mordanted for twenty-four hours with 4 per cent, solution of 

 ferric alum or diluted liq.ferri sulfur, oxydat., then rinsed with water 

 and put for twenty-four hours into an amber-yellow aqueous 

 solution of Kahlbaum's sulfalizarinate of soda, prepared by dropping 

 1 c.c. of saturated alcoholic solution thereof into 80 to 100 c.c. of 

 water. Rinse in water, flood the slides with the solution of crystal 



