CHAPTER XXIX. 351 



743. UNNA' sOrcein Method. (Encyd. mik. Technik., 1910, p. 250). 

 Sections are stained for ten minutes in Griibler's polychrome 

 methylen blue. They are then washed with water, mopped up, 

 and brought for fifteen minutes into a neutral 1 per cent, solution of 

 orcein in absolute alcohol, rinsed in pure alcohol, cleared in bergamot 

 oil, and mounted. Collagenous ground-substance dark red, muscle 

 bluish, elastic fibres sometimes dark red. Material may be fixed in 

 almost any way except with nitric or picric acid, formol, or liquids 

 of Miiller and Hermann. 



744. UNNA'S Methylen-blue -f- Saurefuehsin (UNNA, in Encycl. 

 mik. Technik, 1910, p. 247). Stain for two to five minutes in poly- 

 chrome methylen blue solution (Griibler). Wash and stain for ten 

 to fifteen minutes in " (0-5 per cent.) Saurefuchsin -j- (33 per 

 cent.) tannin-mixture (Griibler)." Water, alcohol, essence, balsam. 

 Collagen, protoplasm, and muscle red, nuclei and keratin blue. On 

 Flemming material, elastin blue. Liquids of Hermann and Erlicki, 

 formol and copper fixatives incompatible. 



745. UNNA'S Safranin -f- Wasserblau (ibid.). Ten minutes in 

 1 per cent, safranin. Wash. Ten to fifteen minutes in " (1 per 

 cent.) Wasserblau + (33 per cent.) tannin mixture." Wash. 

 Stains in opposite colours to the last. Formol and liquid of Hermann 

 contra-indicated for fixing. 



746. Flemming's Orange Method is said to give a very sharp differen- 

 tiation of developing fibrils. 



747. MALLORY (Zeit. wiss. Mik., xviii, 1901, p. 175) stains sections of 

 sublimate or Zenker material lor a few minutes in Saurefuchsin of 0-1 

 per cent, mordants for a few minutes in 1 per cent, phosphomolybdio 

 acid and stains for two to twenty minutes in anilin blue 0-5 grms., 

 Orange G. 2, oxalic acid 2, and water 100. His phosphotungstie 

 haematoxylin stains connective tissue sharply, but does not differentiate 

 it sufficiently from elastic tissue and muscle. 



748. For the complicated procedure of HORNOWSKI see ibid., . xxvi, 

 1909, p. 138. 



749. For DELAMAKE'S mixture or orcein, haematoxylin, Saurefuchsin 

 and picric acid see Verh. Anat. Ges., xix, 1905, p. 227. 



750. MASSON (G. E. Soc. Biol, Ixx, 1911, p. 573), stains first in 

 hsemalum, then in eosin, and then for a, few minutes in 1 per cent, solu- 

 tion of saffron in tap water (made by boiling). Connective tissue, bone, 

 and cartilage, yellow. 



751. Benecke's stain for fibrils (Verh. Anat. Ges., vii, 1893, p. 165) 

 is essentially that of KROMAYER, 716. 



