CHAPTER XXIX. 353 



756. Kresofuchsin (ROTHIG, see 289). 



757. Orcein. This method is due to TAENZEK, and as modified 

 by UNNA is known as the TAENZER-UNNA method, see third edition, 

 or Monatssch. prakt. DermatoL, xii, 1891, p. 394. 



UNNA'S Modified Orcein Method (Monatssch. prakt. DermatoL, 

 xix, 1894, p. 397 ; Zeit. wiss. Mik., xii, 1895, p. 240). Griibler's 

 orcein 1 part, hydrochloric acid 1 part, absolute alcohol 100 parts. 

 Stain sections for thirty to sixty minutes, or for ten to fifteen at 

 30 C., rinse in alcohol, clear, and mount. Elastin dark brown, 

 collagen light brown. 



See also Merle. Sits. Akad. Wiss. Wien., cviii, 1899, p. 335 ; PRANTER, 

 ibid., xix, 1903, p. 361 (lie takes 2 per cent, of nitric acid instead of the 

 hydrochloric, and stains six to twenty-four hours) ; WOLFF, ibid., p. 488 ; 

 the article " Elastin " in Encycl. mik. Technik. ; and E. and T. SAVINI, 

 Zeit. wiss. Mik., xxvi, 1909, p. 34. 



758. WEIGERT'S Resorcin-Fuchsin Method (Centralb. allg. Path., 

 ix, 1898, p. 290). One per cent, of basic fuchsin and 2 per cent, of 

 resorcin (or of carbolic acid) are dissolved in water. Two hundred 

 cubic centimetres of the solution are raised to boiling-point in a 

 capsule, and 25 c.c. of Liquor ferri sesquichlorati P. G. are added, 

 and the whole is boiled, with stirring, for two to five minutes more. 

 A precipitate is formed. After cooling the liquid is filtered, and 

 the precipitate which remains on the filter is brought back into the 

 capsule, and there boiled with 200 c.c. of 94 per cent, alcohol. 

 Allow to cool, filter, make up the filtrate to 200 c.c. with alcohol, 

 and add 4 c.c. of hydrochloric acid. 



WOLFRUM (Zeit. wiss. Mik., xxv, 1908, p. 219) adds 10 to 15 per 

 cent, of acetone to the mixture. 



Stain sections (of material fixed in any way) for twenty minutes 

 to an hour, wash with alcohol, clear with xylol (not with an essence). 

 Elastic fibres dark blue on a light ground. 



MINERVINI (Zeit. wiss. Mik., xviii, 1901, p. 161) gives a variant with 

 safranin instead of fuchsin. 



See also PRANTER, ibid., xix, 1903, p. 361 ; B. FISCHER, Virchow's 

 Arch., clxx, 1902, p. 285, or Zeit. wiss. Mik., xx, 1903, p. 40 (chemistry 

 of the dyes obtained by these processes, which he calls " Fuchselin," 

 " Safranelin," etc.) ; HART, Centralb. allg. Path., xix, 1908, p. 1 ; and 

 CILIMBARIS, Arch. mik. Anat., Ixxv, 1910, p. 708. 



759. Haematoxylin Methods. HARRIS (Zeit. wiss. Mik., xviii, 1902, 

 p. 290) makes an " Elasthrematein " as follows : Haematoxylin 0-2 grms., 

 aluminium chloride 0-1 grms., alcohol of 50 per cent. 100 c.c., boil and 

 add mercuric oxide 0-6 grms., filter and add 1 drop of HC1. Keep for 



M. 23 



