CHAPTER XX XL 409 



RAWITZ (Ztschr. iciss. Mikr., xxvi, 1909, p. 341) has some compli- 

 cated methods with Indulin, 1 'ndaminblau , and Azosdureblau, which take 

 twenty-eight days; and (ibid., xxviii, 1911, p. 1) others with fuchsin 

 and azofuchsiu which take over thirty-six days. 



ARIENS KAPPERS (ibid,, xxviii, 1911, p. 417) describes a staining 

 method with extract of elderberries for material fixed and hardened in 

 Mutter's fluid or similar solutions. It is very simple and particularly 

 recommended for photographic purposes ; it should be carried out as 

 follows : Stain celloidin or paraffin sections overnight in neutralised 

 elderberries extract (obtained by fermentation at 20 to 25 C. ), to which 

 1 per cent, carbolic acid has been added. Wash in water. Differentiate 

 in 3 per cent. Liquor ferri sesquichlorati P.G-., wash, dehydrate, and 

 mount. 



