CHAPTEK XXXIII.* 



MYELIN STAINS. 



852. Iron Haematoxylin. According to A. Bolles Lee (see 1913 Ed.) 

 the simplest way of staining myelin is to make paraffin sections of 

 formol material and stain them with iron hsematoxylin exactly as 

 for central corpuscles (say, twelve to fourteen hours in the mordant, 

 six in the hsematoxylin, and a few minutes for the differentiation). 

 Sections best not over 15 /x. One may counterstain the cells with 

 carmalum, but not for more than half an hour, or the haematoxylin 

 will be attacked. The stain is not so aesthetic as Weigert's, but 

 quite as sharp. Axis cylinders are not shown. 



Similarly REGAUD (C. R. Acad. Sc., cxlviii, 1909, p. 861), but 

 adding a chrome mordantage either concurrently with the formol 

 fixation, or subsequently. Also NAGEOTTE (C. R. Soc. Biol., Ixvii, 

 1909, p. 542), with sections of formol material by the freezing method ; 

 HOUSER (Journ. Comp. Neurol., x, 1901, p. 65), and BROOKOVER 

 (ibid., xx, 1910, No. 2) ; SPIELMEYER (Neurol. CentrbL, xxix, 1910, 

 p. 348) ; and his Technik d. mikrosl. Untersuch. d. Nervensy stems, 

 1911, p. 87, with sections of 25 to 35 /x, by the freezing method ; 

 LOYEZ (C. R. Soc. Biol., Ixix, 1910, p. 511), who differentiates first 

 lightly, till the grey matter begins to appear, in the iron alum, then 

 washes, and differentiates further in Weigert's borax ferricyanide ; 

 GILBERT (Ztsch. wiss. Mikr., xxviii, 1911, p. 279), who mordants with 

 iron alum, stains with molybdic acid hcematoxylin, and differentiates 

 with the borax ferricyanide ; STOELTZNER (ibid., xxiii, 1906, p. 329), 

 who mordants celloidin sections of formol material for five minutes 

 in Liq. ferri sesquichlorati, stains in -5 per cent, hsematoxylin, and 

 differentiates in the mordant or in borax ferricyanide ; and KODIS 

 (Arch. mik. Anat., lix, 1902, p. 211), who fixes for one or two days 

 in saturated solution of mercury cyanide, hardens in 10 per cent, 

 formol, and stains sections, made by the freezing method, with 

 Heidenhain's iron hsematoxylin. 



853. WEIGERT'S Methods*- -There have been in all three methods 

 of WEIGERT: the 1884 method, the 1885 method, and the 1891 

 method. 



* Kevised by Dr. C. Da Fano, King's College, University of London. 



