452 M YE LIN STAINS. 



40 per cent, alcohol, puts them in the dark into 1 per cent, solution of 

 silver nitrate in 40 to 70 per cent, alcohol, then washes thoroughly. 



Similarly, MOSSE (Arch. mik. Anat., lix, 1902, p. 401), impregnating 

 bichromic material with 1 per cent, solution of argentamin, and reducing 

 in 10 per cent, pyrogallic acid, and differentiating by the method of PAL. 



Myelin-and-axis-cylinder Stains. 



875. Methylene Blue. SAHLI (Ztec&r. wiss. MiJcr., ii, 1885, p. 1) stains 

 sections of tissue hardened in bichromate for several hours, in con- 

 centrated aqueous solution of methylene blue, rinses with water, and 

 stains for five minutes in saturated aqueous solution of acid fuchsin. If 

 now the sections are rinsed first with water, then for a few seconds in a 

 1 : 1,000 alcoholic solution of caustic potash, and lastly brought into a 

 large quantity of water, the stain becomes differentiated, axis-cylinders 

 being shown coloured red and the myelin sheaths blue. 



Or (ibid., p. 49), the sections are stained for a few minutes or hours 

 in :- 



Water. . . . . . .40 parts. 



Saturated aqueous solution of methylene 



blue. . . . . . 24 ,, 



5 per cent, solution of borax . . 16 



then washed either in water or alcohol until the grey matter is distinctly 

 differentiated from the white substance, cleared with cedar wood oil, 

 and mounted in balsam. Preparations similar to those obtainable by 

 Weigert's method. 



876. Acid Fuchsin. FINOTTI (Virchow's Archiv., cxliii, 1896, p. 133) 

 stains strongly in Delafield's hsematoxylin, then for a few seconds in 

 concentrated solution of picric acid, then in 0-5 per cent, acid fuchsin, 

 and treats lastly with alkaline alcohol. 



OHLMACHER (Journ. Exper. Med., ii, 1897, p. 675) stains sections for 

 one minute with gentian violet in anilin-water, then for a few seconds in 

 a 0-5 per cent, solution of acid fuchsin in saturated solution of picric acid 

 diluted with 1 volume of water, and differentiates with alcohol and 

 clove oil. 



KAPLAN (Arch. Psychiatr., xxxv, 1902, p. 825) mordants for mouths 

 in Muller, stains sections for a day or more in ^ per cent, aqueous 

 solution of acid fuchsin, rinses in water acidulated with HC1, and 

 differentiates by the method of Pal. 



877. Safranine. ADAMKIEWICZ (Sitzb. Akad. Wiss. Wien. Math. 

 Naturw. Kl., Ixxxix, 1884, Abth. 3, p. 245) stains sections of Muller 

 material in concentrated solution of safranine, differentiates in alcohol 

 and clove oil, brings back again into water, washes in water acidified with 

 acetic acid, and stains in methylene blue. Myelin red, nuclei violet. 



Similarly, CIAGLINSKI (Ztschr. wiss. Mikr., viii, 1891, p. 19) and 

 STROEBE (ibid., x, 1893, p. 384), the former employing safranine followed 

 by anilin blue, whilst the latter first stains with anilin blue, then 

 differentiates with alcohol containing a very little caustic potash, and 

 counters tains with safranine. 



