CHAPTER XXXIV. 477 



the sulphocyanide. (3) Iron method. Sections are kept for thirty 

 seconds in 2 c.c. of a 15 per cent, solution of tannin to which 3 drops of 

 a 5 per cent, solution of oxalic acid have been added. Rinse them first in 

 distilled water and then for a few seconds in 1 per cent, solution of iron 

 chloride until no further blackening occurs. Wash, dehydrate and 

 mount in balsam. Axis -cylinders black, nerve-cells grey. 



APATHY'S Gold Method. See 371. 



GERLACH'S Bichromate and Gold Process. See 369. 



RAMON Y CAJAL'S Gold Method. See Eev. trim. Micr., v, 1900, p. 95. 



UPSON'S Gold and Iron and Vanadium Methods. See MERCIER, 

 Ztschr. wiss. Mikr., vii, 1891, p. 474. 



MAGINI'S Zinc Chloride Process. See Boll. Ace. med. Roma, 1886, or 

 Ztschr. wiss. Mikr., v, 1888, p. 87. 



MONTI'S Copper Process. See Rend. R. Ace. Lincei, Roma, v, 1889, 

 p. 705. 



STRAHUBER'S Anilin Blue Method. See Centrbl. allg. Path., xii, 1901, 

 p. 422. 



CHILESOTTI'S Carmin Stain. See Centrbl. allg. Path., xiii, 1902, p. 191 ; 

 Ztschr. wiss. Mikr., xix, 1902, p. 161, and xx, 1903, p. 87. 



KAPLAN'S Anthracen Ink Method. See Arch. Psych., xxxv, 1902, 

 p. 825. 



MALLORY'S Phosphomolybdic Haematoxylin. See 271. 



DONAGGIO'S Tin Stain. See 273. 



Methylene-blue Methods not considered in Chapter XVI. 



905. S. MEYER'S Method for the Central Nervous System (Arch, 

 mikr. Anat., xlvi, 1895, p. 282, and xlvii, 1896, p. 734). The method 

 consists essentially in injecting animals subcutaneously with large 

 quantities of a solution of methylene-blue B.X., and in treating the 

 central organs (brains) with Bethe's fixing bath. S. Meyer used, at 

 first, a 1 per cent, solution ; later, a solution of methylene-blue B.X. 

 saturated at the body temperature of the animal to be injected 

 (viz., about 5 to 6 per cent.). The injections are to be made at 

 short intervals and in such a way that the animal receives the total 

 quantity it can support in about one to two hours. A cat can 

 support even 150 c.c. ; half-grown rabbits, 30 to 50 c.c. ; fully 

 developed guinea-pigs, 30 to 50 c.c. ; new-born kittens, 15 to 25 c.c. 

 As soon as the animal used is dead, the brain is removed, divided into 

 two to four pieces, and these plunged in 10 per cent, ammonium 

 molybdate to which 1 drop of HC1 for every gram of ammonium 

 molybdate is added. Here they remain for about twenty-four 

 hours at C. Pieces are then washed for two hours in running 

 tap-water, passed quickly through the ascending series of alcohols 

 into absolute alcohol, and, lastly, embedded in paraffin in the usual 

 way. 



