CHAPTER XXXV. 485 



fluid 100 c.c. and sublimate 3 grms., with the addition at the moment 

 of use of acetic acid 3 c.c., formalin 0-5 c.c. The fluid should be 

 warmed at 35 to 40 C. and injected through the blood-vessels, 

 the blood being first washed away by means of Ringer's solution to 

 which 1 : 1000 of amylni trite was added. The tissues are treated 

 in the usual way and embedded in celloidin. The sections 

 are first treated for five minutes with a 1 per cent, solution of 

 caustic soda in 80 per cent, alcohol and washed in distilled water, 

 and then mordanted for a few minutes in 5 per cent, iron alum and 

 washed once more. For staining, Held adds to some distilled water 

 a few drops of a very old molybdic acid hsematoxylin, enough to 

 impart to the water a bluish- violet tone, and stains therein for twelve 

 to twenty-four hours at 50 C. The stain is prepared by dissolving 

 1 grm. of hsematoxylin in 100 c.c. of 70 per cent, alcohol and adding 

 an excess of molybdic acid. Differentiation is carried out by means 

 of the same iron alum solution used for mordanting ; wash well ; 

 counterstain with v. Gieson picro-fuchsin solution ; wash in 96 per 

 cent, alcohol, dehydrate and mount as usual. 



Neuroglia cells and fibres greyish-black ; marginal neuroglia 

 (membrana limitans marginalis and membrana limitans perivascularis) 

 sharply differentiated ; connective tissue pink-red. 



917. Other similar Methods. LHERMITTE and GUCCIONE (Semaine 

 Med., xxix, 1909, p. 205) have the following modification of ANGLADE and 

 MOREL'S method : Sections, made by the freezing method from formalin 

 material, are collected in distilled water and then kept for two hours 

 in a cold -saturated solution of sublimate and for two days in a mixture 

 consisting of 3 parts of 1 per cent, osmic acid, 35 of 1 per cent, chromic 

 acid, 7 of 2 per cent, acetic acid, 55 of distilled water. The rest as 

 Anglade and Morel. 



Similarly MERZBACKER (Journ. Psychol. Neurol., xii, 1909, p. 1). 



DE ALBERTIS (Pathologica, xii, 1920, p. 240) has recently proposed 

 the following combination of the methods of WEIGERT, MALLORY, and 

 ANGLADE and MOREL. Sections are made by means of a freezing 

 microtome from pieces fixed in 15 to 20 per cent, formalin for about 

 twenty-four hours, but not longer than three days. They are transferred 

 into a bath of 2 per cent, acetic acid in 1 per cent, chromic acid (time 

 not stated), and then washed for some hours in repeatedly changed 

 distilled water, oxidised for ten to fifteen minutes in * per cent, 

 potassium permanganate, washed again in distilled water, reduced for 

 fifteen to twenty minutes in 1 per cent, oxalic acid and lastly put to 

 stain for twelve to twenty -four hours in a saturated solution of Victoria 

 blue. For the further treatment sections are washed in distilled water 

 and from this lifted, one by one, by means of a thin glass or platinum 

 spatula, this to be used to plunge each section for an instant first into 

 concentrated LugoFs solution, then into absolute alcohol, and lastly into 

 equal parts of xylol and aniline oil, where the differentiation is accom- 



