498 NEUROGLIA AND SENSE ORGANS. 



has given good results. For the higher vertebrates the injection 

 method should be employed. The Encyd. mik. Technik., i, p. 511, 

 recommends injection of 1 c.c. of 0-5 to 1 per cent, solution 

 every five minutes through the vena femoralis until the death of 

 the animal. The cochlea then to be got out, exposed to the air 

 for fifteen or thirty minutes, and fixed for some hours (overnight) in 

 10 per cent, ammonium molybdate with a little osmic acid. Then 

 decalcified in trichloracetic acid of 5 per cent, with a trace of 

 platinum chloride, washing for twenty-four hours and got into 

 paraffin. 



For fishes and amphibia the immersion method will suffice. 



927. Other Methods. WALDEYER, Strieker's Handb., p. 958 (decalci- 

 fication either in 0-001 per cent, palladium chloride containing 10 per 

 cent, of HC1, or in chromic acid of 0-25 to 1 per cent.). 



URBAN PRITCHARD (Journ. Eoy. Mic. Soc., xii, 1872, p. 380). Decal- 

 cification in 1 per cent, nitric acid. 



LAVDOWSKY ( Arch. mik. Anat., xiii. 1877, p. 497). Fresh tissues (from 

 the cochlea) are treated with 1 per cent, solution of silver nitrate, then 

 washed for ten minutes in water containing a few drops of 0-5 or 1 per 

 cent, osmic acid solution, and mounted in glycerin. 



MAX FLESCH (Arch. mik. Anat., xvi, 1879, p. 300) ; TAFANI (Arch. 

 Hal. de Biol., vi, 1884, p. 207) ; EICHLER ( Abh. math-phys. Cl. Sachs. 

 Ges. Wiss, xviii, 1892, p. 311 ; Zeit. wiss. Mik., ix, 1892, p. 380 (injection 

 of blood-vessels of the labyrinth) ) ; SIEBENMANN (Die Blutgejdsse im 

 Labyrinthe des menschlichen Ohres, Wiesbaden, Bergmann, 1894 ; Zeii. 

 wiss. Mik., xi, 1894, p. 386) ; GRAY (Journ. Anat. Phys., xxxvii, 1903, 

 p. 379) ; SCOTT (ibid., xliii, 1909, p. 329). 



9.28. Olfactive Nerve-endings, Tactile Corpuscles, etc. Besides 

 the gold method, Chapter XVII, and the methylen-blue method, 

 Chapter XVI, the rapid bichromate and silver method of GOLGI 

 should be employed, and for the olfactive mucosa gives the best 

 results. See VAN GEHUCHTEN, La Cellule, vi, 1890, p. 405. For 

 intra-epidermic nerve-endings, besides the methods given in Chap- 

 ter XXVII, the GOLGI method should be employed. According to 

 VAN GEHUCHTEN (La Cellule, ix, 1893, p. 319) it gives much better 

 results than gold methods. He uses the rapid process. For tactile 

 corpuscles, etc., besides the methods given in 721 and 722, see 

 RAMON Y CAJAL'S neuro-fibril methods. 



