522 METHODS FOR INVERTEBRATES. 



LIST (Zeit. wiss. Mik., iv, 1887, p. 211) treats tentacles of Anthea 

 cereus and Sagartia parasitica for ten minutes with a mixture of 

 100 c.c. of sea water with 30 c.c. of Flemming's strong liquid, then 

 washes out for two or three hours in -2 per cent, acetic acid, and 

 teases in dilute glycerin. Picro-carmine may be used for staining. 



981. Nervous system.- -This group is generally held to be refractory 

 to the Golgi impregnation. HAVET, however (La Cellule, xviii, 1901, 

 p. 388), has obtained good results by the rapid method on young 

 specimens of Metridium dianthus. Besides nerve-cells, there are 

 impregnated neuro-muscular cells, gland-cells, and nematocysts. He 

 leaves for five to eight days in the osmic mixture. He has also had 

 good results by the intra vitam methylen blue method (this is also 

 good for nematocysts). So also has GEOSELJ (Arb. Zool. Inst. Univ.- 

 Wien, xvii, 1909, p. 269), adding the dye to the water with the animals 

 till it gives a steel-blue tint. 



982. Zoantharia with Calcareous Skeletons are difficult to deal with 

 on account of the great contractility of the polyps. Sublimate 

 solution, which ought very often to be taken boiling, sometimes 

 gives good results. 



See also Lo BIANCO, loc. cit., p. 446. 



Sections. See 177 and 178, for undecalcified specimens. 



983. The Alcyonaria have also extremely contractile polyps. In 

 a former edition I suggested for their fixation either hot sublimate 

 solution or glacial acetic acid ( 84). S. Lo BIANCO has since recom- 

 mended essentially similar processes. GARBINI (Manuale, p. 151) 

 drenches them with ether, and brings into strong alcohol. 



WILSON (Mitth. Zool. Stat. Neapel, 1884, p. 3) kills Alcyonaria 

 with a mixture of 1 part of strong acetic acid and 2 parts of concen- 

 trated solution of corrosive sublimate, the animals being removed 

 as soon as dead and hardened for two or three hours in concentrated 

 sublimate solution. 



984. Zoantharia and Alcyonaria. BRAUN (Zoo/. Anz., 1886, 

 p. 458) inundates Alcyonium palmatum, Sympodium coralloides, 

 Gorgonia verrucosa, Caryophyllia cyathus, and Palythoa axinellce 

 with a mixture of 20 to 25 c.c. of concentrated solution of sublimate 

 in sea water with 4 to 5 drops of 1 per cent, osmic acid, and after 

 five minutes passes into successive alcohols. 



(This method also gives good results with Hydra and some Bryozoa 

 and Rotifers.) 

 See also S 14. 



