CHAPTER XXXVI. 547 



solution containing 2 per cent, of formol (pure formol produces 

 swellings), or in a mixture of 1 -5 grm. of salt and 250 c.c. of water 

 with the white of an egg. He fixes with sublimate, makes paraffin 

 sections, and stains with hsemalum or iron hsematoxylin. He stains 

 the Sporozoites by making cover-glass preparations, which are 

 allowed to dry, put for twenty-five minutes into absolute alcohol, 

 and stained by the process of ROMANO WSKY, 784, 1008. 



For minute instructions for the application of this process to 

 sections, see GIEMSA, Deutsch. med. Wochenschr., xxxvi, No. 12, 

 1910 ; and SCHUBERG, ibid., xxxv, No. 40, 1909 (Zeit. wiss. Mik., 

 xxvii, 1910, pp. 160, 161 and 513). 



For clinical methods, see COLES, The Diseases of the Blood. London, 

 J. and A. Churchill, 1905. 



BRADFORD and PLIMMER (Quart. Journ. Micr. Sci., xlv, 1902, 

 p. 452) fix Trypanosomes in vapour of equal parts of acetic acid and 

 2 per cent, osmic acid, or with GULLAND'S formol and absolute 

 alcohol, and stain with methylen blue and eosin, and mount in 

 turpentine colophonium. 



HINDLE (Univ. Calif. Pub. Zool., vi, 1909, p. 129) makes smears 

 on cover-glasses coated with albumen, fixes for five minutes in 

 liquid of Flemming, passes through water up to absolute alcohol, 

 then for ten minutes into alcohol of 80 per cent, with a good propor- 

 tion of iodine in potassic iodide, then into 30 per cent, alcohol, and 

 stains with iron hsematoxylin or safranin, then with polychrome 

 methylen blue, and lastly with UNNA'S orange with tannin, and gets 

 quickly through alcohol into xylol and balsam. 



MINCHIN (Quart. Journ. Micr. Sci., liii, 1909, p. 762) makes cover- 

 glass smears, fixes them with vapours of osmic acid (with or without 

 acetic acid), and mounts them dry, or in balsam after fixing in 

 liquids and various stains, amongst these that of TWORT. Half- 

 saturated solutions of neutral red and Lichtgriin are mixed, the 

 precipitate dried and dissolved to about 0-1 per cent, in methyl 

 alcohol with 5 per cent, of glycerin. Three parts of this are diluted 

 with 1 of water, the smears stained for an hour, differentiated with 

 UNNA'S glycerin-ether, and mounted in balsam. This stain works 

 best after fixation with sublimate. 



POLICARD (C. R. Soc. Biol., Ixviii, 1910, p. 505) stains Trypano- 

 somes intra vitam by adding a drop of concentrated solution of neutral 

 red to the edge of a drop of blood spread between slide and cover. 



1033. Flagellata. LAUTERBORN (Zeit. wiss. Zool., lix, 1895 

 p. 170) fixes Ceratium for about ten minutes in liquid of Flemming, 



puts into alcohol for twenty-four hours, brings back into water, 



35 2 



