MACE NATION AND DIGESTION. 315 



has found that STIRLING'S solution greatly deteriorates cellular 

 elements, but that good results are obtained by combining it 

 with a fixing agent. 



The best results were obtained with a 2 per cent, solution 

 of sulphocyanide combined with liquid of RIPART and PETIT. 



SOULIER also obtained good results by combining liquid of 

 RIPART and PETIT with artificial serum of KRONECKER instead 

 of sulphocyanide, or with pepsin, eau de Javelle, 10 per cent, 

 sulphate of soda, or 1'5 per cent, solution of caustic soda. 



And he further found that good results are obtained by 

 combining solutions of chloride of sodium, or solutions of 

 caustic potash or soda, with any of the usual fixing agents. 



528. Saliva, Artificial (for embryology of nerve and muscle) 

 (CALBERLA, Arch. f. mik. Anat., xi, 1875, p. 449). 



Second formula (the first is suppressed) : 



Potassium chloride . . . . 0'4 



Sodium chloride . . . . . 0'3 



Phosphate of soda . . . 0'2 



Calcium chloride ..... 0'2 



1-1 



These are dissolved in 100 parts of water, saturated with 

 carbonic acid, and the solution combined with half a volume 

 of MULLER'S solution and a volume of water. 



The MULLER'S solution may be replaced by a 2| per cent, 

 solution of chromate of ammonia. The best results were 

 obtained when the solutions were saturated with the COo 

 just before using. 



529. LANDOIS'S Solution (Arch. f. mik. Anat., 1885, p. 445). 

 Saturated sol. of neutral chromate of ammonia 5 parts. 

 Saturated sol. of phosphate of potash . . 5 

 Saturated sol. of sulphate of soda . . . 5 ,, 

 Distilled water . . . . . 100 



Small pieces of tissue are macerated for one to three, or 

 even four to five days, in the liquid, then brought for twenty- 

 four hours into ammonia carmine diluted with one volume of 

 the macerating liquid. 



GIERKE particularly recommends this liquid for all sorts of 

 macerations, but especially for the central nervous system, for 



