CHAPTER XXIX. 



MUSCLE AND TENDON (NERVE-ENDINGS). 

 Striated Muscle. 



664. Muscle-cells. For the study of these and allied sub- 

 jects see, inter alia, BEHRENS, KOSSEL, und SCHIEFFERDECKER, 

 Das Mikroskop, etc., vol. ii, pp. 154 161 ; also, for the ap- 

 plication of the gold method to the study of nruscle-cells, 

 SCHAFER, Proc. Roy. Soc., xlix, 1891, p. 280; or Journ. Roy. 

 Mic. Soc., 1891, p. 683. 



Iron hasmatoxylin gives very fine images of striped 

 muscle. 



665. Nerve-endings. For the study of nerve-endings in 

 muscle, both motor and sensory, the four chief methods are 

 the methylen blue method, the gold method, the silver method, 

 and the bichromate of silver method of Golgi. 



666. Nerve-endings the Methylen Blue Method. - - BIEDER- 

 MANN'S procedure for the muscles of Astacus has been indicated 

 in 326 (see also Zeit.f. iriss. Mik., vi, 1, 1889, p. 65). After 

 impregnating as there directed the carapace should be opened, 

 and the muscles exposed to the air in a roomy moist chamber 

 for from two to six hours, in order that the stain may differ- 

 entiate. The abdominal and caudal muscles are those which 

 give the best results. 



For Hydropliilus piceus, BIEDERMANN proceeded by inject- 

 ing 0'5 c.c. of methylen blue solution between the ultimate 

 and penultimate abdominal rings, in the ventral furrow, and 

 keeping the animals alive in water for three to four hours. 

 After this time the thorax should be opened by two lateral 

 incisions, and the muscles of the first pair of legs (which are 



