374 CHAPTER XXIX. 



673. Corpuscles of Golgi (CiACCio, Mem. R. Ace. Sci. Bologna 

 [4], t. x, 1890, p. 301; Zeit. f. wiss. Mik., vii, 4, 1891, 

 p. 507). For Amphibia the usual gold methods are not 

 satisfactory, because the ground- substance of the tendon 

 takes the stain at the same time as the nerve-endings. 

 Pieces of tendon should be put into O'l per cent, hydro- 

 chloric acid or 0'2 per cent, acetic acid until quite trans- 

 parent. They should then be put for five minutes into a 

 mixture of O'l per cent, gold chloride and O'l per cent, 

 potassium chloride. After that they are put back into the 

 acetic acid, and remain there for a day in the dark, and for 

 two or three hours more in the sunlight. When they have 

 become somewhat violet they are put for a day into O'l per 

 cent, osmic acid, and finally mounted in Price's glycerin 

 acidulated with 0'5 per cent, of acetic or formic acid. 



Smooth Muscle. 



674. Test for Smooth Muscle (RETTERER, Complex Rend. Soc. 

 Biol.,iv, 1887, p. 645; Journ. Roy. Mic. Soc., 1888, p. 843). 



If a specimen of tissue be fixed in a mixture of ten 

 volumes of 90 per cent, alcohol and one volume of formic 

 acid, well washed, and stained for twenty-four to thirty-six 

 hours with alum-carmine, the cytoplasm of smooth muscle 

 will be found to be stained red, whilst connective-tissue cells 

 remain unstained, and are swollen. 



675. Smooth Muscle Isolation of Fibres. -- Methods of 

 SCHWALBE, see Arch. f. mik. Anat., 1868, p. 394, or ^yrr/<>* 

 editions. 



GAGE'S methods. See Journ. Roy. Mic. Soc., 1887, p. 327 ; 

 and 525, 537, and 540. 



MOBIUS, liquid for maceration of the muscle of Cardiiim, 

 see above, 536. 



BALLOWITZ, muscle of Cephalopoda, see Arch.f. mik. Auaf., 

 xxxix, 1892, p. 291 ; Zeit. f. wiss. Mik., ix, 3, 1893, p. 344. 



SCHULTZ (Arch. Anat. Phys., Phijs. AltJi., 1895-6, p. 

 521) puts smooth muscle of Vertebrates for twenty-four 

 hours into 10 per cent, nitric acid, rinses with water, and 



