392 CHAPTER XXX. 



(see Ze.it. f. wiss. Mik., xv, 1899, p. 375), and LUXENBURG, 

 NeuroL Centralb., xviii, 1899, p. 629 ; Zeit. f. iviss. Mik., xvi, 

 4, 1900, p. 477. 



698. Toluidin Blue. LENHOSSEK (NeuroL Centralb., xvii, 

 1898, p. 577; Zeit. f. wiss. Mik., xv, 1899, p. 492) thinks 

 that for the study of the corpuscles of Nissl toluidin blue is 

 preferable. Sections are stained on slides for a night in 

 concentrated solution of toluidin blue, rinsed in water, quickly 

 differentiated with alcohol, cleared with xylol or carbolic- acid 

 xylol, and mounted in balsam. They may be very lightly 

 counter-stained with erythrosin before the differentiation. 



Similarly POLDMORDWINOW (Zeit. f. wiss. Mik., xvi, 1899,. 

 p. 371), who stains in a very weak alkaline solution, 1 part 

 of 1 per cent, solution to 119 of water and 1 of carbonate of 

 soda. 



699. Neutral Red. JULICSBURGER (NeuroL Centralb., xvi, 

 1897, p. 259; Zeit. f. wiss. Mik., xiv, 2, 1897, p. 211) stains- 

 sections of formol material for half to three quarters of a 

 minute in warm 1 per cent, solution of neutral red, dehy- 

 drates in alcohol, and passes through bergamot oil to balsam. 



ROSIN (Deutsche med. Wochenschr., 1898, No. 39, p. 615; 

 Zeit. f. wiss. Mik., xvi, 2, 1899, p. 238) stains in concentrated 

 aqueous solution, washes out thoroughly with water, and 

 passes through alcohol (must be free from acid) into xylol 

 and balsam. A double, metachromatic stain, granules of 

 Nissl red, nucleoli red, all the rest yellow. 



700. Methods of KEHM (Munchener med. WochensJir., 1892, No. 13;. 

 Zeit.f. tviss. Mik., ix, 3. 1893, p. 390), see last edition. 



701. WEIGERT'S Stain for Nuclear Figures (Allg. Zeit. f. 

 Psychiatrie, 1, 1894, p. 245). Sections of alcohol material, 

 cut without imbedding, are put for half an hour into Tinct. 

 Perri Acet. Rademacheri, rinsed, stained for a quarter of an 

 hour in 1 per cent, solution of hasmatoxylin in water, rinsed, 

 differentiated rapidly in 70 per cent, alcohol containing 1 per 

 cent, of hydrochloric acid, and mounted in balsam. 



702. Other Methods. See Cox, Zeit.f. wiss. Mik., xiii, 1896, p. 498 ,- 

 xv, 3, p. 369 ; xvi, 1, 1899, p. 101 ; Anat. Hefte, xxxi, 1898, p. 75 ; Intern. 



