410 CHAPTER XXXII. 



appears clearly differentiated from the white to the naked 

 eye. 



733. KAISEK (Zeit.f. iviss. Mik., vi, 4, 1889, p. 471) advises naphthylamin 

 brown (Griibler). Sections of spinal cord are stained for a few hours in a 

 solution containing 1 part of naphthylamin brown, 200 parts of water, and 

 100 parts of alcohol, washed with alcohol, cleared with origanum oil, and 

 mounted. 



734. REHM (Munch, med. Wochenschr., 1892, No. 13 ; Zeit. f. wiss. 

 Mik., ix, 3, 1893, p. 389) gives a method modified from NISSL. Sections of 

 alcohol -hardened material are stained for half a minute to a minute in a 

 hot O'l per cent, solution of methylen blue, washed in 96 per cent, alcohol 

 till no more colour comes away, cleared with origanum oil, and mounted in 

 balsam or benzin-colophonium. Nerve cells, dark blue ; connective-tissue 

 cells lighter, and greenish. For further details see previous editions. 



735. MONCKEBKRG and BETHK (Arcli.f. mik. An at., liv, J899, 

 p. 135; Zeit.f. H-/S.S- Mik., xvi, 1899, p. 244) recommend (for 

 peripheral nerves only) the following : Nerves are fixed in 

 0'25 per cent, osmic acid for twenty-four hours and bleached 

 with bisulphite of sodium, as directed 38, and cut in paraffin. 

 The sections are stained on the slide for ten minutes in O'l 

 per cent, solution of toluidin blue, warmed to 50 or 60 C., 

 washed with water for one or two minutes, then treated for 

 a few seconds or minutes with 1 per cent, solution of 

 molybdate of ammonium. Water, alcohol, xylol, balsam. 



Or the sections are first mordanted for five to ten minutes 

 in 4 per cent, solution of molybdate of ammonium warmed 

 to 20 or 30 C., and washed with water; then toluidin blue 

 solution (of 0*05 to O'l per cent.) is poured on to the slide,, 

 which is put for five minutes into a stove at 50 to 60 C. 

 Water, alcohol, xylol, balsam. 



736. WOLTEE'S Chloride of Vanadium process for axis- 

 cylinder and cell staining' is as follows (Zrit.f. ?n'.y.y. Mik., 

 v'ii, 4, 1891, p. 47]) : 



The material (either central or peripheral nervous tissue) 

 is hardened in tlie Inchromate liquid of KULTSCHITZKY, 59, 

 followed by alcohol, an there described. Sections are mor- 

 danted for twenty-four hours in a mixture of 2 parts of 10 

 per cent, solution of chloride of vanadium and 3 parts of 

 3 per cent, solution of acetate of aluminium, washed for ten 



