434 CHAPTER XXXII. 



elements; but if necessary take 1 per cent, palladium chloride. 

 Make paraffin sections. 



Isolation preparations of the stria vascularis may be made 

 by putting a cochlea for a day into 1 per cent, solution of 

 osmic acid, then for four to five days into O'l per cent, solu- 

 tion ; the stria may then be got away whole. 



784. Other Methods. WALDEYEE, Strieker's Handb., p. 958 (decalci- 

 fication either in O'OOl per cent, palladium chloride containing 10 per cent, 

 of HC1, or in chromic acid of 0'25 to 1 per cent.). 



UEBAN PEITCHABD (Journ. Roy. Mic. Soc., 1876, p. 211). Deealcifica- 

 tion in 1 percent, nitric acid. 



LAVDOWSKY (Arch. f. mik. Anat., 1876, p. 497). Fresh tissues (from 

 the cochlea) are treated with 1 per cent, solution of silver nitrate, then 

 washed for ten minutes in water containing a few drops of 0'5 or 1 per cent, 

 osmic acid solution, and mounted in glycerin. 



MAX FLESCH (Arcli.f. mik. Anat., 1878, p. 300). 



TAPANI (Arch. Hal. de Biol., vi, p. 207). 



POLITZEE, " Die anatomische u. histologische Zergliederung d. mensch- 

 lichen Gehororganes," Stuttgart (Enke), 1889 (see Zeit. f. iviss. Mik., vii, 

 3, 1890, p. 364). 



EICHLEE, Abh. d. math-phys. Cl. d. k. Sdchsischen Ges. d. Wiss., Bd. 

 xviii, 1892, p. 311 ; Zeit. f. iviss. Mik., ix, 3, 1893, p. 380 (detailed 

 account of manipulations for injection of blood-vessels of the labyrinth) ; 

 SIEBENMANN, Die Blutgeftisse im Labyrinthe des menschlichen Ohres, 

 Wiesbaden, Bergmann, 1894; cf. Zeit.f. iviss. Mik., xi, 3, 1894, p. 386. 



Electric Organs. 



785. Torpedo. Raj a. BALLOWITZ (Arch. mik. Anat., xlii, 1893, 

 p. 460) gives a review of all the older methods. He himself 

 gets the best results by the Golgi impregnation, controlled 

 by treatment of fresh tissues for one or two days with osmic 

 acid of 1 per cent, and teasing. 



IWANZOFF (Bull. Soc. Nat. Moscoit, 2, viii, 1895, p. 407) in- 

 jects osmic acid of 0*5 to 2 per cent., removes the pillars after 

 a few minutes, and hardens in 2 per cent, bichromate of 

 potash, stains in hasmatoxylin, and imbeds in paraffin. 



He (ibid., ix, 1895, p. 74) fixes the organ in the tail of 

 Raja with liquid of Flemming, stains, and cuts as above. 



BALLOWITZ (Anat. Hcfte, 1 Abth., vii, 1897, p. 285) finds 

 the method of Golgi excellent for this organ. 



