SOME OTHER HISTOLOG1CAL METHODS. 439 



Heat on a water-bath until reduced to lOO'O. Use for 

 staining undiluted, or diluted with one vol. of anilin water. 

 Differentiate (details not given) with glycol, styron, or creosol. 

 Mastzellen are not differentiated. 



c. Stain giving Red Mastzellen with Blue Plasma Cells. 



Methylen blue . . . I'O 



Kali Carbon, (natron carbon, ammon. 



carbon) . . . .1*0 



Aq. dest. (Aq. carbolisata, chloro- 



forma) .... lOO'O 

 Dilute about 1 00-fold, stain slowly, treat with 70 to 80 per 

 cent, alcohol, differentiate in styron, and bring through ber- 

 gamot oil or xylol into balsam. In this process the granules 

 of the Mastzellen stain red in consequence of the formation 

 of methylen. red in the staining bath. 



See further UNNA in Monatssch. f. prakt. Dermatol., xii, 1891, p. 296 ; 

 Zeit.f. wiss. Mile., ix, 1, 1892, p. 92, and Monatssch. f. prakt. Dermatol., 

 six, 1894, p. 225; Zeit.f. iviss. Mik., xii, 2, 1895, p. 58; also VAX DEE 

 SPEK and UNNA, loc. cit., xiii. 1891, p. 364; Zeit. f. iviss. Mik., xi, 1, 1892, 

 p. 89. 



795. Plasma Cells and Mastzellen. BHJKGONZINI (Anat. Anz. } 

 vi, 1891, pp. 595600; Zeit. f. iviss. MiJc.,ix, 1, 1892, p. 95). 

 Mix 1 volume of 0'2 per cent, solution of Saurefuchsin 

 with 2 vols. of a like solution of methyl green, and 2 vols. 

 of a like solution of gold-orange, and filter through cotton 

 wool. Stain alcohol or sublimate sections (after washing in 

 water) for three to four minutes, wash for one or two 

 minutes in water, bring into absolute alcohol for two minutes, 

 clear in bergamot oil or pure creosote, wash in turpentine, 

 and mount in balsam. 



One sort of gold-orange precipitates methyl green, and 

 therefore cannot be used in this mixture. Orange G- may 

 be used instead. 



796. Plasma Cells. JADASSOHN (Arch. f. Dermatol. u. Syphilis, 

 Erganzungsheft 1, 1892, p. 58 ; Zeit. f. wiss. Mik., ix, 2, 1892, p. 226) re- 

 commends staining for not too long in a 1 : 2000 strongly alkaline or borax 

 solution of thioni", and washing out with acidulated water. 



See also VON MAESCHALKO, Arch. f. Dermatol. u. Syi^hil., xxx, 1895, 



