450 CHAPTER XXXIII. 



a solution of acetic acid, copper acetate, copper chloride, 

 osmic acid, thionin, 1 grin, each, water 400, which, mixed 

 with the blood, fixes and stains in about two minutes. 



808. Stains for Blood. Blood prepared as above can be 

 satisfactorily stained with many of the usual reagents. 



Eosin stains rose-red all parts of blood-corpuscles that con- 

 tain haemoglobin (see WISSOZKY, Arch. f. mik. Anat., 1876, 

 p. 479). 



WISSOZKY (loc. cit.) stains in a solution of equal parts of 

 eosin and alum in 200 parts of alcohol, and then with hasma- 

 toxylin. 



MOORE (The Microscope, 1882, p. 73; Journ. Roy. Mic. Soc-., 

 1882, p. 714) stains for three minutes in a similar solution 

 without the alum, washes, and stains for two minutes in a 1 

 per cent, aqueous solution of methyl green. 



The liquid of Chenzinsky has been given ( 300). It 

 stains nuclei and eosinophilous granules. 



ME REEL'S carmine and indigo-carmine stain has been much 

 recommended, but see 372. 



Fresh (unfixed) blood maybe treated as follows (BizzozERO 

 and TOERE, Archivio per le Scienze mediche, vol. iv, No. 18, 

 1880, p. 390) : Dilute a drop of blood with 0-75 per cent. 

 salt solution in which has been dissolved a little methyl 

 violet. This liquid in no wise affects the form of the ele- 

 ments, stains intensely the nucleus of the red corpuscles, and 

 in the white stains the nucleus intensely, and the protoplasm 

 less intensely. May be used for the study of bone marrow 

 and spleen. 



For the staining of the blood-plates of BIZZOZKRO, this 

 observer (Arch. f. path. Anat. u. Pliys. ; Zeit. f. iviss. Mik., 

 1884, p. 389) employs a 0'02 per cent, solution of methyl 

 violet in salt solution, or a 1 : 3000 solution of gentian violet. 



TOISON (Journ. Sci. wed. de Lille, fev., 1885 ; Zeit. f. iciss. 

 Mile., 1885, p. 398) recommends that blood be mixed with 

 the following fluid : 



Distilled water . . . 160 c.c. 



Glycerin (neutral, 30 Baume) . 30 ,, 

 Pure sulphate of sodium . . 8 grammes. 



Pure chloride of sodium . . 1 gramme. 



Methyl violet 5 B . 0'25 



