SOME OTHER H1STOLOGICAL METHODS. 453 



of balsam and a cover are added. This stain may be applied 

 to celloidin sections without previous removal of the celloidin. 

 See also the modifications of this method by KROMAYER ( 656) 

 and BENECKE ( 786). 



UNNA (Monatsschr. praki. Dermat., xx, 1895, p. 140 ; Zeit.f. iviss. Mik., 

 xiii, 1896, p. 229) gives a modification of the above method, and also one 

 with polychromatic methylen blue and iodide of potassium, and one with 

 fuchsin and tannin. 



WOLFF (Zeit. f. u-iss. Mik., xv, 1899, p. 310) makes up the stain with 

 two vols. saturated solution of carbonate of lithia to one of alcoholic 

 gentian or fuchsin ; other details loc. cit., or Journ. Roy. Mic. Soc., 1899, 

 p. 234. 



Glands. 



811. Mucin. HOYER (Arch. f. mik. Anat., xxxvi, 1890, 

 p. 310; Zeit.f. u-iss. Nik., viii, I, 1891, p. 67) has the follow- 

 ing conclusions : 



The " mucin " of mucus cells and goblet cells stains with 

 basic tar colours and with alum hgematoxylin, but not with 

 acid tar colours (see 262). He obtained his best results 

 by means of thionin, and good ones with toluidin blue, both 

 of these giving a metachromatic stain tissues blue, mucin 

 reddish and also with methylen blue (which is particularly 

 useful from its power of bringing out the merest traces of 

 mucin), safranin, etc. 



All of these colours may be used in the same way. Speci- 

 mens should be fixed for two to eight hours in 5 per cent, 

 sublimate solution, imbedded in paraffin, cut, and the sec- 

 tions stained for five to fifteen minutes in a very dilute 

 aqueous solution of the dye (two drops of saturated solution 

 to 5 c.c, of water). 



It is theoretically interesting to observe that hyaline car- 

 tilage, the jelly of Wharton, and the Mastzellen of Ehrlich 

 give the same reactions with basic dyes as mucin does, even 

 their metachromatic reactions being identical. 



These conclusions had already been in part formulated by 

 SUSSDORP (Deutsche Zeit.f. Thiermed., xiv, pp. 345, 349; see 

 .Zeit.f. u-iss. Mil-., vi, 2, 1889, p. 205). 



See also the series of papers by BIZZOZERO, " Sulle ghian- 

 dole tubidari del tulio gastro-enterico" etc., in the Atti R. 



