SOME OTHER HISTOLOGICAL METHODS. 457 



820. Liver. BRADS (Denkschr. Ned. Nat. Ge. Jena, v, 

 1896, p. 307) demonstrates the bile capillaries by the rapid 

 method of GOLGI, hardening in a mixture of one part formol 

 with three parts liquid of Miiller or 4- per cent, chromic 

 acid. He also stains with Bordeaux R and iron hasma- 

 toxylin, or with Ehrlich-Biondi mixture, after fixing in a 

 mixture of one part formol to three of 7^ per cent, sublimate 

 solution. 



HOLM (Zool. Jahrb., Abth. Morph., x, 1897, p. 283) fixes the extremely 

 fatty liver of Acanihias in a mixture of 5 parts alcohol and 1 of chloroform, 

 and imbeds in paraffin. 



OPI-EL (Anat. Anz., v, 1890, p. 144; vi, 1891, p. 168) puts 

 pieces of liver or spleen (alcohol material) for twenty-four 

 hours into a solution of neutral chromate of potash (i to 

 10 per cent.), rinses with a very weak solution of silver 

 nitrate, puts them for twenty-four hours into a f per cent, 

 solution of silver nitrate, washes, dehydrates and cuts 

 without imbedding. The lattice fibres are only stained near 

 the surface, so that tangential sections must be made. 



See also RANVIER, " Les membranes muqueuses et le syst. glandulaire" 

 Journ. de Microgr., ix, x, 1885-6 ; IGACUSCHI, in Arch. f. path. Anat., 

 xcvii, p. 142, or Zeit.f. wiss. Mik., 1885, p. 243 (gold process for study of 

 fibrous networks) ; KUPFFER, Sitzb. Ges. f. Morph., etc., Munchen, Juli, 

 1889, or Zeit. f. wiss. Mik., vi, 4, 1889, p. 506 (hsematoxylin stain for 

 demonstration of ultimate bile-ducts, and application of Golgi's silver bi- 

 chromate method to the same object and to the study of fibrous networks) ; 

 KBAUSE, Arch. mik. Anat,, xlii, 1893, p. 57. 



821. Spleen. For the lattice fibres, see OPPEL, last . 

 KTJLTSCHITZKY (Arch. inik. Anat., xlvi, 1895, p. 675) studies 



the musculature in sections (of material from liquid of Miiller) 

 stained for a day or more in a solution of " lakmoid " in ether 

 and mounted in balsam. 



For elastic fibres he puts sections for half an hour or a 

 day into a mixture of 800 parts 96 per cent, alcohol, 40 parts 

 1 per cent, solution of carbonate of potash, 2 parts Magdala 

 red, and 1 part methylen blue. 



For the blood-veqselts he puts sections of Miiller material 

 for a few minutes into a solution of one or two parts of 

 Saurerubin in 400 parts of 3 per cent, acetic acid, washes 

 -out in 2 per cent, acetic acid, and after stains in a similar 



