SOME METHODS FOR LOWER ANIMALS. 493" 



way 1 per cent, osmic acid, or, for Amcebas, 2 per cent, 

 chromic acid. 



LANSBERG (ibid., p. 336) advises the same reagents, but 

 recommends bringing the organisms into tlie fxing liquid 

 with a pipette. 



For fixation with iodine (KENT) or iodine vapour (OVERTON) 

 see 88. 



For sulphurous acid, 65. 



CATTANEO (Bollettino Scientifico, iii and iv ; Journ. Roy. 

 Mic. Soc., 1885, p. 538) recommends fixing for a few minutes 

 with ^ per cent, solution of chloride of palladium, which 

 hardens in a few minutes without blackening. 



BRASS (Zeit. f. wiss. Mik., i, 1884, p. 39) employs the follow- 

 ing liquid : 



Chromic acid . . . .1 part. 



Platinum chloride . . 1 ,, 



Acetic acid . . . 1 



Water . . .400 to 1000 parts. 



CERTES (Connies rend., Ixxxviii, 1879, p. 433) fixes with 2 per cent. 

 (sic) osrnic acid, or its vapours (10 to 30 minutes). For details see- 

 previous editions. 



Du PLESSIS (Voox et YUNG, Traite Anat. Comp. Prat., p. 92) recom- 

 mends fixation with 0'2 per cent, solution of corrosive sublimate. Let the 

 preparation dry up, and if the organisms have preserved their shape, stain 

 and mount in balsam. This seemingly barbarous mode of procedure is. 

 said to give very fine preparations when successful. 



FOL (Lehrb., p. 102) fixes delicate marine Infusoria (Tin- 

 tinnodea] with the perchloride of iron solution ( 80), added 

 to the water containing them, and stains with gallic acid as 

 directed, 362, and states that this is the only method that 

 has given him good results, especially as regards the preser- 

 vation of cilia. 



Lo BIANCO (loc. cit., p. 444) fixes Gregarinae with picro-sul- 

 phuric acid (one hour), Vorticelke with hot sublimate, 

 Acinetae with sublimate in sea water, or with osmic acid, 

 Thalassicola with 0'5 per cent, chromic acid (one hour), 

 Acanthometrpe and Aulacanthas with 50 per cent, alcohol or 

 with concentrated sublimate, or by adding a little osmic acid 

 to the water. For Sphaarozoa he proceeds as BRANDT, below. 



ZOGRAF fixes Rhizopoda and Infusoria as Rotatoria, 853, 

 but without narcotisation. 



