118 CHAPTER VIII. 



convenient support on which to gurn the imbedded objects with warm 

 gelatin his artificial pith, thus made : gelatin swollen in water is thoroughly 

 shaken up with i to ^ its volume of castor oil, and the mixture poured into 

 a capsule when just on the point of cooling; the castor oil being then 

 extracted by means of 90 per cent, alcohol, the gelatin remains behind as a 

 finely-porous inelastic mass. 



APATHY (ibid., xii, 1897, p. 718) saturates objects with thin glycerin, 

 gelatin, allows the water to evaporate from it in a desiccator kept just at 

 the melting temperature of the mass, imbeds in metal squares, 126, 

 hardens in absolute alcohol, and cuts under the same. 



145. KLEBS' Gelatin (Glycerin Jelly) (Arch. f. mik. Anat., 

 v, 1869, p. 165). A concentrated solution of isinglass is 

 mixed with half its volume of glycerin. 



146. KAISER'S Gelatin (JBot. Centralb., i, 1880, p. 25; Jo-urn. 

 Roy. Mic. Soc., iii, 1880, p. 504). One part by weight of the 

 finest French gelatin is left for about two hours in 6 parts by 

 weight of water ; 7 parts of glycerin are added, and for 

 every 100 grms. of the mixture 1 grin, of concentrated car- 

 bolic acid. The whole -is warmed for ten to fifteen minutes, 

 stirring all the while, until the whole of the flakes produced 

 by the carbolic acid have disappeared. Filter whilst warm 

 through the finest spun glass, which has been previously 

 washed in water and laid whilst wet in the funnel. 



147. GERLACH'S Gelatin (GKRLACH, Unters. a.d.Anat.Inat. 

 Erlangen, 1884; Jourti. Eoij. Mic. Soc., 1885, p. 541). Take 

 gelatin, 40 grms. ; saturated solution of arsenious acid, 200 

 c.c. ; glycerin, J20 c.c. Clarify with white of egg. The 

 mass may be kept for years in a well-stoppered bottle. The 

 objects to be prepared for imbedding by a bath of one third 

 glycerin. 



148. BRUNOTTI'S Gold Gelatin Mass (Journ. de Satan., vi, 

 1892, p/194; Jo-urn. Roy. Mic. Sac., 1892, p. 706). Twenty 

 grms. gelatin dissolved with heat in 200 c.c. distilled water, 

 and 30 to 40 c.c. of glacial acetic acid with 1 grin, corrosive 

 sublimate added after filtering. At the temperature of 15 C. 

 the mass has the consistence of a thick syrup. Objects are 

 prepared by soaking in some of the mass diluted with two 

 to three vols. of water, then imbedded in the undiluted mass. 



