SERIAL SECTION MOUNTING. 145 



wins. Milt., vii, 4, 1891, p. 457) that after a time the mix- 

 ture loses its adhesive properties, and should be thrown 

 away (G-RANDIS also \_Atti Accad. Liitcri, Send. (4), vi, 1890, 

 p. 138; Arch. Ital. Biol., xiv, 1891, p. 412] states that the 

 albumen of the mixture decomposes after a time). That is 

 not my experience. I find the liquid either first becomes 

 milky, then altogether turbid, and at last coagulates, passing 

 into a caseous state, or it may undergo a hyaline coagulation, 

 drying up like amber. But up to the very last it does not 

 in the least degree lose its adhesive properties. As long as 

 there is enough moisture in it to moisten the brush, I have 

 always found it to stick as well as the first day. 



184. MANX'S Albumen Method (Zeit. f. u-iss. Mik.. xi, 4, 1894, 

 p. 486). Shake up white of egg with ten volumes of distilled water and 

 filter twice through the same paper. Spread this on a stock of slides with 

 a glass rod ; let them drain and dry. Arrange and expand the sections 

 thereon by the water method, 182, put the slide for five minutes on a 

 stove heated to 35 C., then treat with xvlol and alcohol. 



185. SCHAXLIBATTM'S Collodion Method (Arch. f. mil;. Anat., xxii. 

 1883, p. 565). One part of collodion is shaken up with three to four 

 volumes (according to the consistency of the collodion) of clove oil or 

 lavender oil. This should give a clear solution. A little is spread thinly 

 on a slide with a small brush. After arranging the sections on the pre- 

 pared surface, warm over a water-bath, gently, until the clove oil ha.- 

 evaporated (five to ten minutes). The sections are then found to be fixed, 

 and, it is said, can be treated for days with turpentine, chloroform, alcohol, 

 and watery fluids, without becoming detached, thus allowing staining on 

 the slide. According to my experience, however, the method is certainly 

 not safe for that purpose, and should be discarded in favour of the water or 

 the albumen method, and should only be used for already stained sections. 

 See, however, further details in previous editions, or EABL (Zeit.f. wiss. 

 Mik., xi, 2, p. 179); FIELD and MAETIX (Bull. Soc. Zool. de France, xix. 

 189-1, p. 48) ; GALLEMAEBTS (Bull. Soc. Beige de Micro., xv, 1889, p. 56; 

 Zeit. f. iviss. Mik., vi, p. 4, 1889, p. 493); SUMMERS (Amer. Man. Mir. 

 Journ., 1887, p. 73; Zeit.f. wiss. Mik., iv, 4, 1887, p. 482); STEASSEE 

 (Zeit.f. iviss. Mik., iv, 1, 1887, p. 45). 



186. STEASSEE'S Collodion-Paper Method (Zeit.f. wiss. Mik., iii, 3, 

 1886, p. 346). This is an extremely complicated modification of Weigert's 

 method for celloidin sections, and is only adapted for use witli STEASSER'S 

 automatic ribbon-microtome. See the original papers in Zeit.f. iviss.Mik., 

 iii, 3, 1886, p. 346; vi, 2, 1889, p. 154 ; vii, 3, 1890, p. 290; ibid., p. 304 ; 

 ix, 1, 1892, p. 8 ; Journ. Eoij. Mic. Soc., 1892, p. 703 ; Zeit.f. iviss. Mik.. 



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