184 CHAPTER XIII. 



of staan. It is ;i perhaps even more precise nuclear stain, 

 and the solution keeps better. 



240. MAYER'S Glychsemalum (Mittli. Zool. Stat. Neapel, xii, 

 2, 1896, p. 310). Ha-niiitcin (or ha?mateate of ammonia) 

 0'4 grin. (to be rubbed up in a few drops of glycerin in a 

 mortar till it dissolves) ; alum, ."> grms. ; glycerin, 30; dis- 

 tilled water, 70. The stain is not purely nuclear, but may 

 IK- made so by washing out with alum solution or a weak 

 a. -id (^ -2-\C), 238). The solution keeps admirably. 



241. HAXSEX'S Solution (Zool. Anz., 1895, p. 158). See last edition. 

 Hansen oxidises a mixture of alum raid hsematoxylin by means of per- 

 manganate of potash. He fancies that by destroying all germs that may be 

 present in the ingredients, the permanganate should make the solution keep 

 better than the usual solutions. As a matter of fact it does not; mine 

 formed a pellicle and strong precipitate in a few days, and the same was 

 found at the Naples station I.MAYER, in litt.}. See further the remarks of 

 M A\ 1:1: on this process in Mifth. Zool. Stat. Neapel, xii, 18SI6. p. 3o<), or the 

 Grundziige, p. 153. 



242. HARRIS'S Solution iJ/-,-. Hull., xv, 1898, p. 47; Jonrn. Boy. Mic. 

 Soc., ISli'.t. |,. !>:!<;). Alum-hsematoxylin solution ripened by addition of 

 mercuric oxide. 



243. Bohmer's Hsematoxylin (Arch.f. mile. Anat., iv, 1868, p. 345 ; 

 Aii-:.l. Iiili'lliyenzbl., Baimi, 1865, p. 382). Make (A) a solution of 

 ha-matox. crvst. 1 part, alcohol (absolute) 12 parts and (B) alum 1 part, 

 water 2 lu. For staining, add two or three drops of A to a watch-glassful 

 of B. 



The alcoholic solution of hajmatoxylin ought to be old and brown (. 232)- 

 I .-onsider this stain to be of merely historical interest. 



244. Delafield's Hsematoxylin (Zcit. f. wiss. Hik., ii, 1885, p. 288 : 

 frequently attributed erroneously to GKENACHEEOI- PBUDDEK). To 400 c.c. 

 of >;ii mated solution of ammonia-alum* add 4 grms. of hsematox. cryst. 

 li-x.lved in 25 c.c. of strong alcohol. Leave it exposed to the light and air 

 in an uu-toppered bottle for three or four days. Filter, and add luo c.c. of 

 serin and KlO c.c. of methylic alcohol (CH 4 0). Allow the solution to 

 -land until the colour is sufficiently dark, then filter and keep in a tightly 



stoppered buttle. 



Tliis solution keeps well, -it maybe said to keep for years. It is well to 

 allow it to ripen for at least two months before using it. 



I'or staining, enough of the solution should be added to pure water to 



Ammonia-alum dissolves in about 11 parts of water. 



