H^MATEIN (H^MATOXYLIN) STAINS. 191 



2^ per cent, solution of ferric alum, not weuker. Leave the sections 

 therein (fixed to slides hy the water method, 182) for six to twelve hours, 

 or at least not less than three. Keep the slides upright in the mordant, not 

 lying flat. Wash out well with water before staining. Stain in a " ripened " 

 hsematoxylin solution, i. e. one that has stood for four weeks (of course if 

 you make it up with the ripened brown alcoholic solution recommended 

 232, sub fin., this will be superfluous). Stain for twenty-four to thirty- 

 six hours. Use the same staining solution over and over again until it 

 becomes spoilt; for the solution after having been used gives a more ener- 

 getic stain, owing to its containing a trace of iron brought over by the 

 sections. Differentiate in a 2i per cent, solution of ferric alum. Rinse for 

 ten minutes in running water, clear with xylol, not with any essential oil, 

 and mount in xylol-balsam. See further on this subject under " Cytological 

 Methods." 



256. Iron Haematoxylin (BuTSCHLi, Unters. ilber mikrosTcopische 



Schdume u. das Proioplasma, etc., 1892 ; Zeit.f.wiss. Mik., ix, 2, 1892, 

 p. 197). Sections treated with a weak brown aqueous solution of ferric 

 acetate, washed with water, and stained in 05 per cent, aqueous solution of 

 hsematoxylin. This treatment gives a blue-black or brown-black stain of 

 extiaordinary intensity. The process was used by Biitschli for staining 

 sections. 1 /i in thickness, of Protozoa. It does not appear to be of general 

 applicability. 



257 JANSSENS' Iron Haematoxylin (" He'matoxyline noire ; " La 

 Cellule, xiv, 1, 1897, p 207). A similar mixture to that of DELAFIELD, 

 244, ferric alum being taken instead of ammonia alum, the rest as in 

 Delafield's. A progressive stain, nuclear. 



258. BENDA'S Copper Haematoxylin (Arcli.f. mile. Anat., xxx, 1887, 

 p. 49). See last edition. According to my experience, not to be compared 

 with iron hsematoxylin, and superfluous. 



259. MALLOEY'S Phospho-molybdie Acid Haematoxylin (Anat. 

 Anzeig., 1891, p. 375 ; see also Zeit. f. wiss. Mik., viii, 3, 1891, p. 341). 

 One part of 10 per cent, phospho-rnolybdic acid solution, 1 part hsematoxylin, 

 100 parts water, and 6 to 10 parts chloral hydrate. Let the solution ripen 

 for a week in sunlight, and filter. This stain is recommended for prepara- 

 tions of central nervous system, but has been found useful in other cases. 

 Sections should be stained for from 10 minutes to 1 hour, and washed out in 

 two or three changes of 40 to 50 per cent, alcohol. Dehydrate and mount 

 as usual. Celloidin remains colourless. The stain is blue, and in its general 

 effect something like a nigrosin stain. Besides ganglion-cells and glia-cells, 

 axis-cylinders are stained, also many other tissue-elements. It is necessary 

 that the solution be saturated with hsernatoxylin in order to obtain the best 

 results ; if a good stain be not obtained at once, more hsematoxylin must be 

 added. 



See also RIBBEET (Centralb.f. allg. Path., vii, 1896, p. 427 ; Zeit. f. iviss. 

 Mile., xv, 1, 1898, p. 93). 



